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Monthly Archives: March 2014
ABRFers Bid Farewell to Albuquerque
From the opening session to the time our team broke down the Sage Science booth to go home, the ABRF conference was a terrific event. We really enjoyed the big data keynote from Phil Bourne and moderated session that followed. … more »
Cross-Platform Sequencing Studies in the Spotlight at ABRF
It’s day two of the ABRF meeting, and a session comparing next-gen sequencing platforms had attendees riveted. The room was packed for “ABRF Next Generation Sequencing Study,” which featured speakers including George Grills, Don Baldwin, Scott Tighe, Christopher Mason, and … more »
Live from Albuquerque, It’s Protein Day at ABRF!
The annual ABRF meeting kicked off today, and some of the earliest talks focused on proteomics. The Sage team was glad about that, as our newest product, the SageELF, works with proteins as well as DNA. The conference’s opening keynote … more »
As ABRF Approaches, Sage Science Honors Core Lab Customers
With the annual ABRF conference rapidly approaching, we wanted to take a moment to thank the many core lab teams who have adopted Pippin instruments for automated DNA size selection. Some of these customers have independently conducted studies of size … more »
All Aboard for ABRF!
The Sage Science team is gearing up for our favorite core facility meeting: the annual conference of the Association of Biomolecular Resource Facilities. This year the event will be held in sunny Albuquerque, New Mexico, home of the largest hot … more »
Detection of 1,25-Dihydroxyvitamin D-Regulated miRNAs in Zebrafish by Whole Transcriptome Sequencing
March 2014 (epub ahead of print) Authors: Theodore A. Craig, Yuji Zhang, Andrew T. Magis, Cory C. Funk, Nathan D. Price, Stephen C. Ekker, and Rajiv Kumar Info: In this paper from the journal Zebrafish, scientists report a study of … more »
Posted in Citation
Tagged miRNA, miRNA isolation, NEBNext, Pippin Prep
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DNA methylation is required for the control of stem cell differentiation in the small intestine
March 2014 Authors: Karyn L. Sheaffer, Rinho Kim, Reina Aoki, Ellen N. Elliott, Jonathan Schug, Lukas Burger, Dirk Schubeler, and Klaus H. Kaestner Info: Scientists in Switzerland and Pennsylvania sought to understand the role of methylation in stem cell-directed regeneration … more »
Posted in Citation
Tagged DNA Sequencing, Illumina, Pippin Prep
Comments Off on DNA methylation is required for the control of stem cell differentiation in the small intestine
Redefining the Human Oral Mycobiome with Improved Practices in Amplicon-based Taxonomy: Discovery of Malassezia as a Prominent Commensal
March 2014 Authors: Amanda K. Dupuy, Marika S. David, Lu Li, Thomas N. Heider, Jason D. Peterson, Elizabeth A. Montano, Anna Dongari-Bagtzoglou, Patricia I. Diaz, Linda D. Strausbaugh Info: In this PLoS One paper, scientists from the University of Connecticut … more »
Posted in Citation
Tagged 454, amplicon sequencing, Pippin Prep
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Comparison of single-molecule sequencing and hybrid approaches for finishing the genome of Clostridium autoethanogenum and analysis of CRISPR systems in industrial relevant Clostridia
March 2014 Authors: Steven D Brown, Shilpa Nagaraju, Sagar Utturkar, Sashini De Tissera, Simón Segovia, Wayne Mitchell, Miriam L Land, Asela Dassanayake, and Michael Köpke Info: Scientists generated a closed, high-quality genome sequence for a Clostridium autoethanogenum strain capable of … more »
Posted in Citation
Tagged BluePippin, DNA Sequencing, PacBio
Comments Off on Comparison of single-molecule sequencing and hybrid approaches for finishing the genome of Clostridium autoethanogenum and analysis of CRISPR systems in industrial relevant Clostridia
Comprehensive profiling of the vaginal microbiome in HIV positive women using massive parallel semiconductor sequencing
March 2014 Authors: Adam Ameur, Tracy L. Meiring, Ignas Bunikis, Susana Ha¨ggqvist, Cecilia Lindau, Julia Hedlund Lindberg, Inger Gustavsson, Zizipho Z. A. Mbulawa, Anna-Lise Williamson and Ulf Gyllensten Info: Researchers at Uppsala University, Sweden, along with the University of Cape … more »
Posted in Citation
Tagged BluePippin, DNA Sequencing, Ion Torrent
Comments Off on Comprehensive profiling of the vaginal microbiome in HIV positive women using massive parallel semiconductor sequencing