Improved Workflow for HiFi Sequencing
The PippinHT was designed to run two SBS-compatible gel cassettes, each with a 12-sample capacity, and with onboard sample tracking capability. In this way, DNA size selection could more closely match sample prep workflows undertaken on 96-well microplates. However, high molecular weight DNA size selection for single-molecule sequencing has not been optimal in this regard because HMW size selections require an external marker for calibration. This effectively reduces the sample capacity of PippinHT cassettes from 12 samples to 10 for a maximum capacity of 20 samples per run.
Since PacBio recommends the PippinHT for size selection of its HiFI WGS libraries, we developed a cassette kit that will use internal standards to calibrate size selection at the recommended 10kb threshold. This cassette will increase the capacity of the HiFi size selections to the intended 12 samples per cassette, and hopefully better compliment workflows. As an extra bonus, users can expect slightly better accuracy that is achieved with internal markers as well. The Femtopulse and Pippin Pulse gel images below illustrate similar performance to the current external marker method.
If the need arises, we can produce internal standard cassettes with higher Hi-Pass thresholds as well. So if you are running HiFi WGS, or have any application that will benefit from >10kb Hi-Pass, give this cassette a try!
Here is the ordering information: HIJ-7510, 10/pk