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- Whole genome sequencing identifies associations for nonsyndromic sagittal craniosynostosis with the intergenic region of BMP2 and noncoding RNA gene LINC01428
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Tag Archives: PippinHT
Whole genome sequencing identifies associations for nonsyndromic sagittal craniosynostosis with the intergenic region of BMP2 and noncoding RNA gene LINC01428
April 2024 Authors: Anthony M. Musolf, Cristina M. Justice, Zeynep Erdogan-Yildirim, Seppe Goovaerts, Araceli Cuellar, John R. Shaffer, Mary L. Marazita, Peter Claes, Seth M. Weinberg, Jae Li, Craig Senders, Marike Zwienenberg, Emil Simeonov, Radka Kaneva, Tony Roscioli, Lorena Di … more »
Posted in Citation
Tagged oxford nanopore, PippinHT, Promethion
Comments Off on Whole genome sequencing identifies associations for nonsyndromic sagittal craniosynostosis with the intergenic region of BMP2 and noncoding RNA gene LINC01428
Mature microRNA-binding protein QKI promotes microRNA-mediated gene silencing
February 2024 Authors: Kyung-Won Min, Myung Hyun Jo, Minseok Song, Ji Won Lee, Min Ji Shim, Kyungmin Kim, Hyun Bong Park,Shinwon Ha, Hyejin Mun, Ahsan Polash, Markus Hafner, Jung-Hyun Cho, Dongsan Kim, Ji-Hoon Jeong, Seungbeom Ko, Sungchul Hohng, Sung-Ung Kang … more »
Posted in Citation
Tagged miRNA isolation, PippinHT
Comments Off on Mature microRNA-binding protein QKI promotes microRNA-mediated gene silencing
In vitro size-selection of short circulating tumor DNA fragments from late-stage lung cancer patients enhance the detection of mutations and aneuploidies
January 2024 Authors: Christoffer Trier Maansson, Louise Skov Thomsen, Laura Stokkebro, Julie Gabe Dissing, Maiken Parm Ulhoi, Anders Lade Nielsen, Peter Meldgaard, Boe Sandahl Sorensen Abstract: “Introduction Recent studies have demonstrated differences between the fragment length profiles of cell-free DNA … more »
“Range+T “ for Tight Sizing of HMW Libraries
Range+T Size Selection Note: Range + T is available for all PippinHT instruments. However, only BluePippins with serial numbers 2700 and above will run the method. Sage’s DNA size selection technology has been used to help improve PacBio sequencing, particularly … more »
Posted in Blog
Tagged BluePippin, Diagenode, HiFi, Megaruptor, PacBio, PippinHT, Range+T
Comments Off on “Range+T “ for Tight Sizing of HMW Libraries
AGBT23 Roundup!
Well, we have another fabulous AGBT behind us that we were happy to attend. Our take home messages? A. Spatial transcriptomics is the exciting new frontier. B. True human WGS has been accomplished and is moving into population-scale work. And … more »
Utility of long-read sequencing for All of Us
January 2023 Authors: M. Mahmoud, Y. Huang, K. Garimella, P. A. Audano, W. Wan, N. Prasad, R. E. Handsaker, S. Hall, A. Pionzio, M. C. Schatz, M. E. Talkowski, E. E. Eichler, S. E. Levy, F. J. Sedlazeck Abstract: “The … more »
Posted in Citation
Tagged HiFi, PacBio, PippinHT, whole genome sequencing
Comments Off on Utility of long-read sequencing for All of Us
High-molecular weight DNA extraction, clean-up and size selection for long-read sequencing
July 2021 Authors: Ashley Jones ,Cynthia Torkel,David Stanley,Jamila Nasim,Justin Borevitz,Benjamin Schwessinger Info: The authors provide a protocol for preparing high molecular weight DNA sequencing libraries with a particular focus on difficult plant and fungal tissues, and also can be used … more »
Posted in Citation
Tagged oxford nanopore, PacBio, PippinHT
Comments Off on High-molecular weight DNA extraction, clean-up and size selection for long-read sequencing
Gene by environment interaction mouse model reveals a functional role for 5-hydroxymethylcytosine in neurodevelopmental disorders
May 2021 Authors: Ligia A. Papale, Andy Madrid, Qi Zhang, Kailei Chen, Lara Sak, Sündüz Keles, and Reid S. Alisch Info: Researchers studying the role of 5-Hydroxymethylcytosine (5hmC) epigenetic modifications in neurological disorders report on the effect of prenatal stress … more »
Circulating miRNA diversity, origin and response to changing metabolic and reproductive states, new insights from the rainbow trout
Authors: Emilie Cardona, Cervin Guyomar, Thomas Desvignes3, Jérôme Montfort , Samia Guendouz, John H. Postlethwait , Sandrine Skiba-Cassy, Julien Bobe Info: The researchers conducted a comprehensive study of circulating miRNAs (c-mRNAs) in biological fluids of the rainbow trout, with the … more »
Resetting of 24-nt siRNA landscape is initiated before the first zygotic division in rice
January 2021 Authors: Chenxin Li, Jonathan I. Gent, Hengping Xu, Hong Fu, Scott D. Russell, and Venkatesan Sundaresan Info: In this preprint, the authors report on a study of siRNA profiling in zygotes from the rice plant. They report that … more »