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Monthly Archives: July 2015
Biomarker discovery: quantification of microRNAs and other small non-coding RNAs using next generation sequencing
July 2015 Authors: Juan Pablo Lopez, Alpha Diallo, Cristiana Cruceanu, Laura M. Fiori, Sylvie Laboissiere, Isabelle Guillet, Joelle Fontaine, Jiannis Ragoussis, Vladimir Benes, Gustavo Turecki, and Carl Ernst Info: Scientists from McGill University and the European Molecular Biology Laboratory evaluated … more »
Posted in Citation
Tagged Illumina, Pippin Prep
Comments Off on Biomarker discovery: quantification of microRNAs and other small non-coding RNAs using next generation sequencing
Arterial endothelial methylome: differential DNA methylation in athero-susceptible disturbed flow regions in vivo
July 2015 Authors: Yi-Zhou Jiang, Elisabetta Manduchi, Christian J. Stoeckert, and Peter F. Davies Info: In this paper, scientists from the University of Pennsylvania used complete methylated DNA immunoprecipitation sequencing (MeDIP-seq) to study methylation differences in endothelial cells to find … more »
Posted in Citation
Tagged Illumina, Pippin Prep
Comments Off on Arterial endothelial methylome: differential DNA methylation in athero-susceptible disturbed flow regions in vivo
A method to simultaneously construct up to 12 differently sized Illumina Nextera long mate pair libraries with reduced DNA input, time, and cost
July 2015 Authors: Darren Heavens, Gonzalo Garcia Accinelli, Bernardo Clavijo, and Matthew Derek Clark Info: Scientists from The Genome Analysis Centre report a protocol using the SageELF to improve library preparation for long mate-pair sequencing. Using a Nextera kit, they … more »
Scientists Optimize Mate-Pair Sequencing with SageELF
In a Biotechniques paper this month, scientists from The Genome Analysis Centre describe a new method for mate-pair sequencing that saves time and money while decreasing the amount of input DNA required. The method is based on SageELF, which automatically … more »
Posted in Blog
Tagged BluePippin, Methods, SageELF, TGAC
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Towards allele-level human leucocyte antigens genotyping – assessing two next-generation sequencing platforms: Ion Torrent Personal Genome Machine and Illumina MiSeq
June 2015 Authors: J. L. Duke, C. Lind, K. Mackiewicz, D. Ferriola, A. Papazoglou, O. Derbeneva, D. Wallace, and D. S. Monos Info: This publication in the International Journal of Immunogenetics from scientists at the Children’s Hospital of Philadelphia presents … more »
Posted in Citation
Tagged HLA, Illumina, Ion Torrent, Pippin Prep
Comments Off on Towards allele-level human leucocyte antigens genotyping – assessing two next-generation sequencing platforms: Ion Torrent Personal Genome Machine and Illumina MiSeq
Assembly and diploid architecture of an individual human genome via single-molecule technologies
June 2015 Authors: Matthew Pendleton, Robert Sebra, Andy Wing Chun Pang, Ajay Ummat, Oscar Franzen, Tobias Rausch, Adrian M Stütz, William Stedman, Thomas Anantharaman, Alex Hastie, Heng Dai, Markus Hsi-Yang Fritz, Han Cao, Ariella Cohain, Gintaras Deikus, Russell E Durrett, … more »
Posted in Citation
Tagged BluePippin, PacBio
Comments Off on Assembly and diploid architecture of an individual human genome via single-molecule technologies
Hybrid Single-Molecule Approach Produces Highly Contiguous Diploid Human Genome Assembly
A team of scientists from the Icahn School of Medicine at Mount Sinai, Weill Cornell Medical College, Cold Spring Harbor Laboratory, European Molecular Biology Laboratory, and other institutions published the first analysis of a diploid human genome produced by combining … more »
Posted in Blog
Tagged BluePippin, PacBio
Comments Off on Hybrid Single-Molecule Approach Produces Highly Contiguous Diploid Human Genome Assembly