Author Archives: Alex

Identifying the best PCR enzyme for library amplification in NGS

Posted on April 26, 2024 by Alex

April 2024 Authors: Michael A. Quail, Craig Corton, James Uphill, Jacqueline Keane and Yong Gu Abstract: “PCR amplification is a necessary step in many next-generation sequencing (NGS) library preparation methods. Whilst many PCR enzymes are developed to amplify single targets … more »

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Whole genome sequencing identifies associations for nonsyndromic sagittal craniosynostosis with the intergenic region of BMP2 and noncoding RNA gene LINC01428

Posted on April 26, 2024 by Alex

April 2024 Authors: Anthony M. Musolf, Cristina M. Justice, Zeynep Erdogan-Yildirim, Seppe Goovaerts, Araceli Cuellar, John R. Shaffer, Mary L. Marazita, Peter Claes, Seth M. Weinberg, Jae Li, Craig Senders, Marike Zwienenberg, Emil Simeonov, Radka Kaneva, Tony Roscioli, Lorena Di … more »

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Mature microRNA-binding protein QKI promotes microRNA-mediated gene silencing

Posted on February 26, 2024 by Alex

February 2024 Authors: Kyung-Won Min, Myung Hyun Jo, Minseok Song, Ji Won Lee, Min Ji Shim, Kyungmin Kim, Hyun Bong Park,Shinwon Ha, Hyejin Mun, Ahsan Polash, Markus Hafner, Jung-Hyun Cho, Dongsan Kim, Ji-Hoon Jeong, Seungbeom Ko, Sungchul Hohng, Sung-Ung Kang … more »

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In vitro size-selection of short circulating tumor DNA fragments from late-stage lung cancer patients enhance the detection of mutations and aneuploidies

Posted on January 26, 2024 by Alex

January 2024 Authors: Christoffer Trier Maansson, Louise Skov Thomsen, Laura Stokkebro, Julie Gabe Dissing, Maiken Parm Ulhoi, Anders Lade Nielsen, Peter Meldgaard, Boe Sandahl Sorensen Abstract: “Introduction Recent studies have demonstrated differences between the fragment length profiles of cell-free DNA … more »

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The haplotype-resolved T2T carnation (Dianthus caryophyllus) genome reveal the correlation between genome architecture and gene expression

Posted on December 19, 2023 by Alex

November 2023 Authors: Lan Lan, Luhong Leng, Weichao, Yonglin Ren, Wayne Reeve, Xiaopeng Fu, Zhiqiang Wu, Xiaoni Zhang Abstract: “Carnation (Dianthus caryophyllus) is one of the most valuable commercial 45 flowers, due to its richness of colour and form, and … more »

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“Range+T “ for Tight Sizing of HMW Libraries

Posted on August 3, 2023 by Alex

Range+T Size Selection Note: Range + T is available for all PippinHT instruments. However, only BluePippins with serial numbers 2700 and above will run the method. Sage’s DNA size selection technology has been used to help improve PacBio sequencing, particularly … more »

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Cut the Big Stuff: >50kb High Pass DNA Size Selection on the HLS2

Posted on August 1, 2023 by Alex

We have been getting quite a few inquiries of late from researchers who would like to size select DNA fragments above 50kb. This may be due in no small part to our friends at Grandomics, China, who have been citing … more »

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The draft genome sequence of the Japanese rhinoceros beetle Trypoxylus dichotomus septentrionalis towards an understanding of horn formation

Posted on June 6, 2023 by Alex

May 2023 Authors: Shinichi Morita, Tomoko F. Shibata, Tomoaki Nishiyama, Yuuki Kobayashi, Katsushi Yamaguchi, Kouhei Toga, Takahiro Ohde, Hiroki Gotoh, Takaaki Kojima, Jesse N. Weber, Marco Salvemini, Takahiro Bino, Mutsuki Mase, Moe Nakata, Tomoko Mori, Shogo Mori, Richard Cornette, Kazuki … more »

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Targeted Phasing of 2-200 Kilobase DNA Fragments with a Short-Read Sequencer and a Single-Tube Linked-Read Library Method

Posted on March 6, 2023 by Alex

March 2023 Authors: Veronika Mikhaylova, Madison Rzepka, Tetsuya Kawamura1, Yu Xia, Peter L. Chang, Shiguo Zhou, Long Pham, Naisarg Modi, Likun Yao, Adrian Perez-Agustin, Sara Pagans, T. Christian Boles , Ming Lei, Yong Wang, Ivan Garcia-Bassets, and Zhoutao Chen Abstract: … more »

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AGBT23 Roundup!

Posted on February 22, 2023 by Alex

Well, we have another fabulous AGBT behind us that we were happy to attend.   Our take home messages? A. Spatial transcriptomics is the exciting new frontier.  B.  True human WGS  has been accomplished and is moving into population-scale work. And … more »

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