Comparison of single-molecule sequencing and hybrid approaches for finishing the genome of Clostridium autoethanogenum and analysis of CRISPR systems in industrial relevant Clostridia

March 2014

Steven D Brown, Shilpa Nagaraju, Sagar Utturkar, Sashini De Tissera, Simón Segovia, Wayne Mitchell, Miriam L Land, Asela Dassanayake, and Michael Köpke

Scientists generated a closed, high-quality genome sequence for a Clostridium autoethanogenum strain capable of fermenting CO, CO2, and H2 into biofuels or industrial chemicals. They used BluePippin to collect fragments 4 Kb and greater for sequencing on the PacBio® platform. Among novel findings was the discovery that the organism has a CRISPR system, which may make it more amenable to use in biotechnology than closely related strains without that system.

Biotechnology for Biofuels 2014, 7:40


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