Short-read DNA sequencers have trouble identifying large structural variants. These could be in the form of copy number variants, translocations, duplications, deletions, insertions, and inversions. The TELL-Seq™ WGS Library Prep kit links short-reads turning them into long-reads. The linked, long-read data that you get using the TELL-Seq™ kit will allow you to take a step back and see what you were missing.
Knowing which homologous chromosome has which sequence is essential to understanding a genome. Whole Genome Phasing is difficult with 2nd generation, short-read, DNA sequencing technology. Phasing provides haplotype information which is important for understanding complex traits and variant linkages. The TELL-Seq™ WGS DNA Library Prep kit helps with this by linking short-reads to larger fragments and therefore long-reads.
HLS-CATCH™; Genomic Target Analysis
With the SageHLS™; electrophoresis platform, Cas9 enzymology can be used to purify larger genomic regions from cell preparations. Using the TELL-Seq™ small genome option, these targets – up to 500 Mb in length – can be sequenced for structural variant analysis, haplotype phasing, or de novo assembly. Targets can selected by user-designed guideRNAs, or predesigned panels developed by Sage Science may be used.
De Novo Assembly
Using the TELL-Seq™ WGS library prep kit you can sequence novel genomes. This works for small bacterial genomes, medium sized insect, plant, and fungi genomes and large animal and plant genomes. The TELL-Seq™ kit will turn an ordinary short-read 2nd generation DNA sequencer into a long-read 3rd generation DNA sequencer. If you really want to understand a new genome, including gene synteny, you must look at long-reads.
Samples containing disparate microbes are essential to gaining insight into our ecosystem as well as human disease. The TELL-Seq™ WGS Library Prep kit will allow you to sequence samples at a metagenomic scale; providing long-read like results with the ease of short-read sequencing. To see what is truly in a mixed microbial sample use the power of long-read DNA sequencing with the simplicity of short-read sequencing.