Detection of 1,25-Dihydroxyvitamin D-Regulated miRNAs in Zebrafish by Whole Transcriptome Sequencing

March 2014 (epub ahead of print)

Theodore A. Craig, Yuji Zhang, Andrew T. Magis, Cory C. Funk, Nathan D. Price, Stephen C. Ekker, and Rajiv Kumar

In this paper from the journal Zebrafish, scientists report a study of microRNAs regulating gene expression induced by the presence of vitamin D in zebrafish larvae. They found that the number of miRNAs increases significantly after a week of development and hypothesize that this is due to organ and other complexity. They used Pippin Prep together with the NEBNext Multiplex Small RNA Kit from New England Biolabs to enrich for miRNAs in the sample.

Zebrafish 2014


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