Monthly Archives: March 2016

New hyRAD Method Expands Use of RAD-seq to Degraded Samples

Since RAD-seq was first developed, we’ve seen a number of new versions and approaches from an enthusiastic scientific community. The latest was recently published in PLoS One and demonstrates a RAD-based method suitable for analyzing degraded DNA, an essential step … more »

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Hybridization Capture Using RAD Probes (hyRAD), a New Tool for Performing Genomic Analyses on Collection Specimens

March 2016 Authors: Tomasz Suchan, Camille Pitteloud, Nadezhda S. Gerasimova, Anna Kostikova, Sarah Schmid, Nils Arrigo, Mila Pajkovic, Michał Ronikier, and Nadir Alvarez Info: Scientists present a new protocol based on the RAD-seq concept that works more effectively for samples … more »

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Identification of Human Islet Amyloid Polypeptide as a BACE2 Substrate

February 2016 Authors: Ingrid C. Rulifson, Ping Cao, Li Miao, David Kopecky, Linda Huang, Ryan D. White, Kim Samayoa, Jonitha Gardner, Xiaosu Wu, Kui Chen, Trace Tsuruda, Oliver Homann, Helene Baribault, Harvey Yamane, Tim Carlson, Jed Wiltzius, Yang Li Info: … more »

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Pitfalls of haplotype phasing from amplicon-based long-read sequencing

February 2016 Authors: Thomas W. Laver, Richard C. Caswell, Karen A. Moore, Jeremie Poschmann, Matthew B. Johnson, Martina M. Owens, Sian Ellard, Konrad H. Paszkiewicz & Michael N. Weedon Info: University of Exeter researchers used long-range PCR and long-read sequencing … more »

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Comparison of Sample Preparation Methods Used for the Next-Generation Sequencing of Mycobacterium tuberculosis

February 2016 Authors: Andrea D. Tyler, Sara Christianson, Natalie C. Knox, Philip Mabon, Joyce Wolfe, Gary Van Domselaar, Morag R. Graham, and Meenu K. Sharma Info: In this PLoS One publication, scientists in Manitoba compare the quality of libraries constructed … more »

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The Many Dimensions of Diet Breadth: Phytochemical, Genetic, Behavioral, and Physiological Perspectives on the Interaction between a Native Herbivore and an Exotic Host

February 2016 Authors: Joshua G. Harrison, Zachariah Gompert, James A. Fordyce, C. Alex Buerkle, Rachel Grinstead, Joshua P. Jahner, Scott Mikel, Christopher C. Nice, Aldrin Santamaria, and Matthew L. Forister Info: In this study, scientists analyzed butterflies colonizing several different … more »

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Experimental feline enteric coronavirus infection reveals an aberrant infection pattern and shedding of mutants with impaired infectivity in enterocyte cultures

January 2016 Authors: Lowiese M. B. Desmarets, Ben L. Vermeulen, Sebastiaan Theuns, Nádia Conceição-Neto, Mark Zeller, Inge D. M. Roukaerts, Delphine D. Acar, Dominique A. J. Olyslaegers, Marc Van Ranst, Jelle Matthijnssens, and Hans J. Nauwyncka Info: Scientists in Belgium … more »

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Transcriptome profiling of Kentucky bluegrass (Poa pratensis L.) accessions in response to salt stress

January 2016 Authors: B. Shaun Bushman, Keenan L. Amundsen, Scott E. Warnke, Joseph G. Robins, and Paul G. Johnson Info: Scientists compared the transcriptomes of two strains of Kentucky bluegrass, one sensitive to salt and the other tolerant even to … more »

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