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- Complete and haplotype-specific sequence assembly of segmental duplication-mediated genome rearrangements using targeted CRISPR-targeted ultra-long read sequencing (CTLR-Seq)
- Ultra-low input single tube linked-read library method enables short-read second-generation sequencing systems to generate highly accurate and economical long-range sequencing information routinely generate highly accurate and economical long-range sequencing information
- CRISPR–Cas9/long-read sequencing approach to identify cryptic mutations in BRCA1 and other tumour suppressor genes
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Category Archives: Citation
ATAC-seq with unique molecular identifiers improves quantification and footprinting
November 2020 Authors: Tao Zhu, Keyan Liao, Rongfang Zhou, Chunjiao Xia & Weibo Xie Info: The authors suggest an improvement on the ATAC-seq method (Assay for Transposase-Accessible Chromatin with high-throughput sequencing). By using unique molecular identifiers to distinguish between transposase … more »
Complete and haplotype-specific sequence assembly of segmental duplication-mediated genome rearrangements using targeted CRISPR-targeted ultra-long read sequencing (CTLR-Seq)
October 2020 Authors: Bo Zhou, GiWon Shin, Stephanie U. Greer, Lisanne Vervoort, Yiling Huang, Reenal Pattni, Marcus Ho, Wing H. Wong, Joris R. Vermeesch, Hanlee P. Ji, Alexander E. Urban Info: In this preprint the authors report on a method, … more »
Posted in Citation
Tagged HLS-CATCH, MinION, oxford nanopore, SageHLS
Comments Off on Complete and haplotype-specific sequence assembly of segmental duplication-mediated genome rearrangements using targeted CRISPR-targeted ultra-long read sequencing (CTLR-Seq)
Ultra-low input single tube linked-read library method enables short-read second-generation sequencing systems to generate highly accurate and economical long-range sequencing information routinely generate highly accurate and economical long-range sequencing information
June 2020 Authors: Zhoutao Chen, Long Pham, Tsai-Chin Wu, Guoya Mo, Yu Xia, Peter L Chang, Devin Porter, Tan Phan, Huu Che, Hao Tran, Vikas Bansal, Justin Shaffer, Pedro Belda-Ferre, Gregory Humphrey, Rob Knight, Pavel Pevzner, Son Pham, Yong Wang … more »
Posted in Citation
Tagged TELL-Seq
Comments Off on Ultra-low input single tube linked-read library method enables short-read second-generation sequencing systems to generate highly accurate and economical long-range sequencing information routinely generate highly accurate and economical long-range sequencing information
CRISPR–Cas9/long-read sequencing approach to identify cryptic mutations in BRCA1 and other tumour suppressor genes
October 2020 Authors: Tom Walsh, Silvia Casadei, Katherine M Munson, Mary Eng, Jessica B Mandell, Suleyman Gulsuner, Mary-Claire King Info: In this short report, researchers studying young-onset breast cancer used the SageHLS and HLS-CATCH method to purify high molecular weight … more »
A long-read RNA-seq approach to identify novel transcripts of very large genes
July 2020 Authors: Prech Uapinyoying, Jeremy Goecks, Susan M. Knoblach, Karuna Panchapakesan, Carsten G. Bonnemann, Terence A. Partridge, Jyoti K. Jaiswal, and Eric P. Hoffman Info: Scientists researching muscular disorders present a method for studying gene expression of large RNA … more »
Posted in Citation
Tagged BluePippin, iso-seq, PacBio
Comments Off on A long-read RNA-seq approach to identify novel transcripts of very large genes
Fully phased sequence of a diploid human genome determined de novo from the DNA of a single individual
July 2020 Authors: Ilya Soifer , Nicole L. Fong, Nelda Yi, Andrea T. Ireland, Irene Lam, Matthew Sooknah, Jonathan S. Paw, Paul Peluso, Gregory T. Concepcion, David Rank, Alex R. Hastie, 5 Vladimir Jojic, J. Graham Ruby, David Botstein, Margaret … more »
Posted in Citation
Tagged BluePippin, High Pass, PacBio
Comments Off on Fully phased sequence of a diploid human genome determined de novo from the DNA of a single individual
Highly accurate long-read HiFi sequencing data for five complex genomes
May 2020 Authors: Ting Hon, Kristin Mars, Greg Young, Yu-Chih Tsai, Joseph W. Karalius, Jane M. Landolin, Nicholas Maurer, David Kudrna, Michael A. Hardigan, Cynthia C. Steiner, Steven J. Knapp, Doreen Ware, Beth Shapiro, Paul Peluso, David R. Rank Info: … more »
Posted in Citation
Tagged BluePippin, HiFi, PacBio, SageELF
Comments Off on Highly accurate long-read HiFi sequencing data for five complex genomes
Decimated little brown bats show potential for adaptive change
April 2020 Authors: Giorgia G. Auteri & L. Lacey Knowles Info: Little brown bats have experienced a 90% population decline due to fungal pathogen that causes a disease, white-nose syndrome. The authors used ddRADseq to compare surviving and non-surviving individuals. … more »
Posted in Citation
Tagged ddRADseq, Pippin Prep
Comments Off on Decimated little brown bats show potential for adaptive change
A restriction enzyme reduced representation sequencing approach for low-cost, high-throughput metagenome profiling
April 2020 Authors: Melanie K. Hess, Suzanne J. Rowe, Tracey C. Van Stijn, Hannah M. Henry, Sharon M. Hickey, Rudiger Brauning, Alan F. McCulloch, Andrew S. Hess, Michelle R. Kirk, Sandeep Kumar, Cesar Pinares-Patiño, Sandra Kittelmann, Graham R. Wood, Peter … more »
Posted in Citation
Tagged GBS, Pippin Prep
Comments Off on A restriction enzyme reduced representation sequencing approach for low-cost, high-throughput metagenome profiling
Inhalation of lung spheroid cell secretome and exosomes promotes lung repair in pulmonary fibrosis
February 2020 Authors: Phuong-Uyen C. Dinh, Dipti Paudel, Hayden Brochu, Kristen D. Popowski, M. Cyndell Gracieux, Jhon Cores, Ke Huang, M. Taylor Hensley, Erin Harrell, Adam C. Vandergriff, Arianna K. George, Raina T. Barrio, Shiqi Hu, Tyler A. Allen, Kevin … more »
Posted in Citation
Tagged microRNA, Pippin Prep
Comments Off on Inhalation of lung spheroid cell secretome and exosomes promotes lung repair in pulmonary fibrosis