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- Telomere-to-telomere reference genome reveals subgenome divergence and large structural variants in Capsella bursa-pastoris (Brassicaceae)
- Integrated analysis of miRNAs and mRNAs in thousands of single cells
- Cost-effective and Flexible Preimplantation Genetic Testing (PGT) Using Adaptive Sampling-based Targeted Nanopore Sequencing (ASTN-seq)
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Category Archives: Citation
Chasing non-existent “microRNAs” in cancer
April 2025 Authors: Ayla Orang, Nicholas I. Warnock, Melodie Migault, B. Kate Dredge, Andrew G. Bert, Julie M. Bracken, Philip A. Gregory, Katherine A. Pillman, Gregory J. Goodall & Cameron P. Bracken Abstract: “MicroRNAs (miRNAs) are important regulators of gene … more »
Posted in Citation
Tagged cancer, miRNA, Pippin Prep
Comments Off on Chasing non-existent “microRNAs” in cancer
Telomere-to-telomere reference genome reveals subgenome divergence and large structural variants in Capsella bursa-pastoris (Brassicaceae)
March 2025 Authors: Xin Liu, Lu Liu, Hai-Fei Yan, Xue-Jun Ge, Hui-Run Huang Abstract: “Capsella is a model genus for studying polyploid speciation and mating system transitions. Here, we report a subgenome-resolved telomere-to-telomere reference genome of Capsella bursa-pastoris, the only … more »
Posted in Citation
Tagged HMW DNA, oxford nanopore, Plant, SageHLS
Comments Off on Telomere-to-telomere reference genome reveals subgenome divergence and large structural variants in Capsella bursa-pastoris (Brassicaceae)
Integrated analysis of miRNAs and mRNAs in thousands of single cells
January 2025 Authors: Jia Li, Jing Tian & Tao Cai Abstract: “The simultaneous sequencing of multiple types of biomolecules can facilitate understanding various forms of regulation occurring in cells. Cosequencing of miRNA and mRNA at single-cell resolution is challenging, and … more »
Posted in Citation
Tagged miRNA, Pippin Prep, RNA-seq
Comments Off on Integrated analysis of miRNAs and mRNAs in thousands of single cells
Cost-effective and Flexible Preimplantation Genetic Testing (PGT) Using Adaptive Sampling-based Targeted Nanopore Sequencing (ASTN-seq)
January 2025 Authors: Zhiqiang Zhang, Shujing He, Taoli Ding, Xiaoyan Liang, Cong Fang, Haitao Zeng, Linan Xu, Xiaolan Li, Lei Jia, Shihui Zhang, Wenlong Su, Peng Sun, Ji Yang, Jun Ren, Sijia Lu, Zi Ren Abstract: “Genetic diseases encompass a … more »
Posted in Citation
Tagged Adaptive Sequencing, BluePippin, ONT, oxford nanopore
Comments Off on Cost-effective and Flexible Preimplantation Genetic Testing (PGT) Using Adaptive Sampling-based Targeted Nanopore Sequencing (ASTN-seq)
CFTR haplotype phasing using long-read genome sequencing from ultra-low input DNA
January 2025 Authors: Neeru Gandotra, Antariksh Tyagi, Irina Tikhonova, Caroline Storer, Curt Scharfe Abstract: “Purpose: Newborn screening (NBS) identifies rare diseases that result from the recessive inheritance of pathogenic variants in both copies of a gene. Long-read genome sequencing (LRS) … more »
Integrated analysis of miRNAs and mRNAs in thousands of single cells
January 2025 Authors: Jia Li, Jing Tian, Tao Cai Abstract: “The simultaneous sequencing of multiple types of biomolecules can facilitate understanding various forms of regulation occurring in cells. Cosequencing of miRNA and mRNA at single-cell resolution is challenging, and to … more »
Posted in Citation
Tagged miRNA, mRNA, Pippin Prep, single cell sequencing
Comments Off on Integrated analysis of miRNAs and mRNAs in thousands of single cells
Leveraging the power of long reads for targeted sequencing
December 2024 Authors: Shruti V. Iyer, Sara Goodwin, and William Richard McCombie Abstract: “Long-read sequencing technologies have improved the contiguity and, as a result, the quality of genome assemblies by generating reads long enough to span and resolve complex or … more »
Posted in Citation
Tagged CATCH, HLS-CATCH, long reads, long-read sequencing, Target Enrichment
Comments Off on Leveraging the power of long reads for targeted sequencing
Resolving the 22q11.2 deletion using CTLR-Seq reveals chromosomal rearrangement mechanisms and individual variance in breakpoints
July 2024 Authors: Bo Zhou, Carolin Purmann, Hanmin Guo, GiWon Shin, Yiling Huang, Reenal Pattni, Qingxi Meng, Stephanie U. Greer, Tanmoy Roychowdhuryg, Raegan N. Wood, Marcus Ho, Heinrich zu Dohna, Alexej Abyzov, Joachim F. Hallmayer, Wing H. Wong, Hanlee P. … more »
CTLR-Seq Protocol
July 2024 Authors: Bo Zhou, GiWon Shin,Yiling Huang, Raegan N. Wood, Hanlee P. Ji, Alexander E. Urban Abstract: “We developed a generally applicable method CRISPR/Cas9-targeted long read sequencing (CTLR-Seq) to resolve, haplotype-specifically, and at base-pair resolution, large, complex, and highly … more »
Processing frozen archival human DNA samples for large-scale SQK-LSK114 Oxford Nanopore long-read DNA sequencing SOP v1
June 2024 Authors: Alicia Wenghöfer, Kimberly Paquette, Laksh Malik, Breeana Baker, Cedric Kouam, Kimberley J Billingsley Abstract: “As part of the GP2 monogenic network we will generate long-read sequencing data to better understand the genetic architecture of Parkinson’s disease. To … more »
Posted in Citation
Tagged BluePippin, ONT, oxford nanopore
Comments Off on Processing frozen archival human DNA samples for large-scale SQK-LSK114 Oxford Nanopore long-read DNA sequencing SOP v1