-
Recent Posts
- Resolving the 22q11.2 deletion using CTLR-Seq reveals chromosomal rearrangement mechanisms and individual variance in breakpoints
- CTLR-Seq Protocol
- Processing frozen archival human DNA samples for large-scale SQK-LSK114 Oxford Nanopore long-read DNA sequencing SOP v1
- Identifying the best PCR enzyme for library amplification in NGS
- Whole genome sequencing identifies associations for nonsyndromic sagittal craniosynostosis with the intergenic region of BMP2 and noncoding RNA gene LINC01428
Tags
10x Genomics ABRF agbt Amplicon ASHG ASMS BluePippin ChIP-Seq ddRADseq DNA Sequencing Featured genotyping High Pass HiSeq HLA HLS HLS-CATCH Illumina ILMN Ion Torrent isoseq Mate-pair Sequencing Methods microRNA miRNA miRNA isolation MiSeq ONT oxford nanopore PacBio PAG Paired-end Sequencing PippinHT Pippin Prep Pippin System podcast Protein Mass Spec Protein Size Fractionation proteomics RAD-seq RNA-seq RNA sequencing SageELF SageHLS Tips and ApplicationsRecent Comments
- Alex on And Now, a Word from Our Customer Service Department
- Donna on And Now, a Word from Our Customer Service Department
- Tony Gill on And Now, a Word from Our Customer Service Department
- Alex on And Now, a Word from Our Customer Service Department
- Andrea on And Now, a Word from Our Customer Service Department
Archives
- July 2024
- June 2024
- April 2024
- February 2024
- January 2024
- December 2023
- August 2023
- June 2023
- March 2023
- February 2023
- January 2023
- November 2022
- September 2022
- April 2022
- January 2022
- September 2021
- July 2021
- June 2021
- May 2021
- February 2021
- January 2021
- November 2020
- October 2020
- July 2020
- May 2020
- April 2020
- March 2020
- February 2020
- January 2020
- November 2019
- August 2019
- July 2019
- June 2019
- May 2019
- April 2019
- March 2019
- February 2019
- January 2019
- December 2018
- October 2018
- September 2018
- May 2018
- April 2018
- March 2018
- February 2018
- January 2018
- December 2017
- November 2017
- October 2017
- September 2017
- August 2017
- July 2017
- June 2017
- May 2017
- April 2017
- March 2017
- February 2017
- January 2017
- December 2016
- November 2016
- October 2016
- September 2016
- August 2016
- July 2016
- June 2016
- May 2016
- April 2016
- March 2016
- February 2016
- January 2016
- December 2015
- November 2015
- October 2015
- September 2015
- August 2015
- July 2015
- June 2015
- May 2015
- April 2015
- March 2015
- February 2015
- January 2015
- December 2014
- November 2014
- October 2014
- September 2014
- August 2014
- July 2014
- June 2014
- May 2014
- April 2014
- March 2014
- February 2014
- January 2014
- December 2013
- November 2013
- October 2013
- September 2013
- August 2013
- July 2013
- June 2013
- May 2013
- April 2013
- March 2013
- February 2013
- January 2013
- December 2012
- November 2012
- October 2012
- September 2012
- August 2012
- July 2012
- June 2012
- May 2012
- April 2012
- March 2012
- February 2012
- December 2011
- October 2011
- September 2011
- June 2011
- February 2011
- November 2010
- October 2010
- August 2010
Tag Archives: BluePippin
Processing frozen archival human DNA samples for large-scale SQK-LSK114 Oxford Nanopore long-read DNA sequencing SOP v1
June 2024 Authors: Alicia Wenghöfer, Kimberly Paquette, Laksh Malik, Breeana Baker, Cedric Kouam, Kimberley J Billingsley Abstract: “As part of the GP2 monogenic network we will generate long-read sequencing data to better understand the genetic architecture of Parkinson’s disease. To … more »
Posted in Citation
Tagged BluePippin, ONT, oxford nanopore
Comments Off on Processing frozen archival human DNA samples for large-scale SQK-LSK114 Oxford Nanopore long-read DNA sequencing SOP v1
Identifying the best PCR enzyme for library amplification in NGS
April 2024 Authors: Michael A. Quail, Craig Corton, James Uphill, Jacqueline Keane and Yong Gu Abstract: “PCR amplification is a necessary step in many next-generation sequencing (NGS) library preparation methods. Whilst many PCR enzymes are developed to amplify single targets … more »
Posted in Citation
Tagged BluePippin, PCR, SageELF
Comments Off on Identifying the best PCR enzyme for library amplification in NGS
“Range+T “ for Tight Sizing of HMW Libraries
Range+T Size Selection Note: Range + T is available for all PippinHT instruments. However, only BluePippins with serial numbers 2700 and above will run the method. Sage’s DNA size selection technology has been used to help improve PacBio sequencing, particularly … more »
Posted in Blog
Tagged BluePippin, Diagenode, HiFi, Megaruptor, PacBio, PippinHT, Range+T
Comments Off on “Range+T “ for Tight Sizing of HMW Libraries
Optimising ddRAD sequencing for population genomic studies with ddgRADer
October 2022 Authors: Aparna Lajmi, Felix Glinka, Eyal Privman Info: In this preprint, the authors describe an online webtool that they have developed for designing ddRAD-seq experiments. This webtool assists in the recommendation of restriction enzyme pairs and size-selection criteria. … more »
Posted in Citation
Tagged BluePippin, ddRADseq, Pippin Prep
Comments Off on Optimising ddRAD sequencing for population genomic studies with ddgRADer
Heat selection enables highly scalable methylome profiling in cell-free DNA for noninvasive monitoring of cancer patients
September 2022 Authors: Elsie Cheruba, Ramya Viswanathan, Pui-Mun Wong, Howard John Womersley, Shuting Han, Brenda Tay, Yiting Lau, Anna Gan, Polly S. Y. Poon, Anders Skanderup, Sarah B. Ng, Aik Yong Chok, Dawn Qingqing Chong, Iain Beehuat Tan, Lih Feng … more »
Posted in Citation
Tagged bisulfite sequencing, BluePippin, cell-free DNA, Illumina, methylation
Comments Off on Heat selection enables highly scalable methylome profiling in cell-free DNA for noninvasive monitoring of cancer patients
An experimental strategy for preparing circular ssDNA virus genomes for next-generation sequencing
Feb 2022 Authors: Catherine D. Aimone, Steen Hoyer, Anna E. Dye, David O. Deppong, Siobain Duffy, Ignazio Carbone, Linda Hanley-Bowdoin Info: The authors provide a protocol for analyzing single stranded DNA (ssDNA) from begomoviruses, which cause significant damage to many … more »
Posted in Citation
Tagged BluePippin, ssDNA
Comments Off on An experimental strategy for preparing circular ssDNA virus genomes for next-generation sequencing
High-quality Arabidopsis thaliana Genome Assembly with Nanopore and HiFi Long Reads
September 2021 Authors: Bo Wang, Xiaofei Yang, Yanyan Jia, Yu Xu, Peng Jia, Ningxin Dang, Songbo Wang, Tun Xu, Xixi Zhao, Shenghan Gao, Quanbin Dong, Kai Ye Info: The authors report on a high quality genome assembly of the model … more »
Posted in Citation
Tagged BluePippin, HiFi, HLS, oxford nanopore, PacBio
Comments Off on High-quality Arabidopsis thaliana Genome Assembly with Nanopore and HiFi Long Reads
Comparison of long-read sequencing technologies in interrogating bacteria and fly genomes
June 2021 Authors: Eric S Tvedte, Mark Gasser, Benjamin C Sparklin, Jane Michalski, Carl E Hjelmen, J Spencer Johnston, Xuechu Zhao, Robin Bromley, Luke J Tallon, Lisa Sadzewicz, David A Rasko, Julie C Dunning Hotopp Info: This side-by-side comparison of … more »
Posted in Citation
Tagged BluePippin, oxford nanopore, PacBio
Comments Off on Comparison of long-read sequencing technologies in interrogating bacteria and fly genomes
Nanopore adaptive sampling: a tool for enrichment of low abundance species in metagenomic samples
May 2021 Authors: Samuel Martin, Darren Heavens, Yuxuan Lan, Samuel Horsfield, Matthew D Clark, Richard M Leggett Info: Oxford Nanopore adaptive sampling is a real-time sequencing method with which analysis software identifies several hundred sequence bases as they pass through … more »
Posted in Citation
Tagged BluePippin, High Pass, oxford nanopore
Comments Off on Nanopore adaptive sampling: a tool for enrichment of low abundance species in metagenomic samples
Chromosomal rearrangements but no change of genes and transposable elements repertoires in an invasive forest-pathogenic fungus
Authors: Arthur Demené , Benoît Laurent , Sandrine Cros-Arteil , Christophe Boury , Cyril Dutech Info: The authors present a high-quality de novo genome assembly of Cryphonectria parasitica, and invasive fungus that causes chestnut blight, using Oxford Nanopore and Illumina … more »
Posted in Citation
Tagged BluePippin, GridION, High Pass, High Pass Plus, ONT, oxford nanopore
Comments Off on Chromosomal rearrangements but no change of genes and transposable elements repertoires in an invasive forest-pathogenic fungus