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Tag Archives: oxford nanopore
High-quality Arabidopsis thaliana Genome Assembly with Nanopore and HiFi Long Reads
September 2021 Authors: Bo Wang, Xiaofei Yang, Yanyan Jia, Yu Xu, Peng Jia, Ningxin Dang, Songbo Wang, Tun Xu, Xixi Zhao, Shenghan Gao, Quanbin Dong, Kai Ye Info: The authors report on a high quality genome assembly of the model … more »
Posted in Citation
Tagged BluePippin, HiFi, HLS, oxford nanopore, PacBio
Comments Off on High-quality Arabidopsis thaliana Genome Assembly with Nanopore and HiFi Long Reads
High-molecular weight DNA extraction, clean-up and size selection for long-read sequencing
July 2021 Authors: Ashley Jones ,Cynthia Torkel,David Stanley,Jamila Nasim,Justin Borevitz,Benjamin Schwessinger Info: The authors provide a protocol for preparing high molecular weight DNA sequencing libraries with a particular focus on difficult plant and fungal tissues, and also can be used … more »
Posted in Citation
Tagged oxford nanopore, PacBio, PippinHT
Comments Off on High-molecular weight DNA extraction, clean-up and size selection for long-read sequencing
Comparison of long-read sequencing technologies in interrogating bacteria and fly genomes
June 2021 Authors: Eric S Tvedte, Mark Gasser, Benjamin C Sparklin, Jane Michalski, Carl E Hjelmen, J Spencer Johnston, Xuechu Zhao, Robin Bromley, Luke J Tallon, Lisa Sadzewicz, David A Rasko, Julie C Dunning Hotopp Info: This side-by-side comparison of … more »
Posted in Citation
Tagged BluePippin, oxford nanopore, PacBio
Comments Off on Comparison of long-read sequencing technologies in interrogating bacteria and fly genomes
Nanopore adaptive sampling: a tool for enrichment of low abundance species in metagenomic samples
May 2021 Authors: Samuel Martin, Darren Heavens, Yuxuan Lan, Samuel Horsfield, Matthew D Clark, Richard M Leggett Info: Oxford Nanopore adaptive sampling is a real-time sequencing method with which analysis software identifies several hundred sequence bases as they pass through … more »
Posted in Citation
Tagged BluePippin, High Pass, oxford nanopore
Comments Off on Nanopore adaptive sampling: a tool for enrichment of low abundance species in metagenomic samples
Chromosomal rearrangements but no change of genes and transposable elements repertoires in an invasive forest-pathogenic fungus
Authors: Arthur Demené , Benoît Laurent , Sandrine Cros-Arteil , Christophe Boury , Cyril Dutech Info: The authors present a high-quality de novo genome assembly of Cryphonectria parasitica, and invasive fungus that causes chestnut blight, using Oxford Nanopore and Illumina … more »
Posted in Citation
Tagged BluePippin, GridION, High Pass, High Pass Plus, ONT, oxford nanopore
Comments Off on Chromosomal rearrangements but no change of genes and transposable elements repertoires in an invasive forest-pathogenic fungus
Complete and haplotype-specific sequence assembly of segmental duplication-mediated genome rearrangements using targeted CRISPR-targeted ultra-long read sequencing (CTLR-Seq)
October 2020 Authors: Bo Zhou, GiWon Shin, Stephanie U. Greer, Lisanne Vervoort, Yiling Huang, Reenal Pattni, Marcus Ho, Wing H. Wong, Joris R. Vermeesch, Hanlee P. Ji, Alexander E. Urban Info: In this preprint the authors report on a method, … more »
Posted in Citation
Tagged HLS-CATCH, MinION, oxford nanopore, SageHLS
Comments Off on Complete and haplotype-specific sequence assembly of segmental duplication-mediated genome rearrangements using targeted CRISPR-targeted ultra-long read sequencing (CTLR-Seq)
A spontaneous complex structural variant in rcan-1 increases exploratory behavior and laboratory fitness of Caenorhabditis elegans
February 2020 Authors: Yuehui Zhao, Lijiang Long, Jason Wan, Shweta Biliya, Shannon C. Brady, Daehan Lee, Akinade Ojemakinde, Erik C. Andersen, Fredrik O. Vannberg, Hang Lu, Patrick T. McGrath Info: Researchers studying genetic adaptive changes using C. elegans recombinant inbred … more »
Posted in Citation
Tagged BluePippin, oxford nanopore
Comments Off on A spontaneous complex structural variant in rcan-1 increases exploratory behavior and laboratory fitness of Caenorhabditis elegans
Sequencing of human genomes with nanopore technology
April 2019 Authors: Rory Bowden, Robert W. Davies, Andreas Heger, Alistair T. Pagnamenta, Mariateresa de Cesare, Laura E. Oikkonen, Duncan Parkes, Colin Freema, Fatima Dhalla, Smita Y. Patel, Niko Popitsch, Camilla L.C. Ip, Hannah E. Roberts, Silvia Salatino, Helen Lockstone, … more »
Posted in Citation
Tagged BluePippin, ONT, oxford nanopore, wgs
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London Calling: Who Will Beat the 1 Mb Read?
Later this week, scientists will descend on Old Billingsgate, London, for Oxford Nanopore’s annual user event. Better known as London Calling, the meeting has become famous for talks full of cutting-edge nanopore sequencing results, novel protocols, and best practices. The … more »
App Note: BluePippin Sizing Yields Longer MinION Reads
BluePippin users are generating longer reads from their Oxford Nanopore MinION sequencers, as demonstrated in a new app note from our colleagues at Nippon Genetics. The app note showcases a workflow used by Kazuharu Arakawa, an associate professor in the … more »
Posted in Blog
Tagged BluePippin, MinION, ONT, oxford nanopore
Comments Off on App Note: BluePippin Sizing Yields Longer MinION Reads