Tag Archives: Tips and Applications

What’s the difference between the 4-10 Kb vs2 and 6-10 Kb vs3 high pass protocols?

Customers may have noticed two very similar cassette definitions; 0.75%DF Marker S1 high-pass 4-10kb vs2 and 0.75%DF Marker S1 High-Pass 6-10kb vs3. Since these two protocols cover a similar range, they might seem a bit redundant. However, both of these … more »

Posted in Blog, Tips | Tagged , , | Comments Off on What’s the difference between the 4-10 Kb vs2 and 6-10 Kb vs3 high pass protocols?

Pippin Pulse

Field Inversion Gel Electrophoresis       We’ve set up this post to provide Pippin Pulse users with a resource to keep updated on new protocols and to post references that might be useful. We also invite users to feel … more »

Posted in Blog, Tips and Applications | Tagged , , | Comments Off on Pippin Pulse

And Now, a Word from Our Customer Service Department

Here at Sage Science, we are delighted to see more and more people signing up as customers of the Pippin platform. With so many instruments out in the wild, we thought it would be a good time to sit down … more »

Posted in Blog, Tips and Applications | Tagged , , | 5 Comments

BluePippin “Mid-Range” Size Selection: 18-27 kb

We’re pleased to be shipping our Mid-Range size selection cassette (BMF7510) for the BluePippin. While our Low-Range cassette (BLF7510, 1kb-10kb) works for many mate-pair library construction protocols, we’ve been hearing from our customers that there is interest in creating larger … more »

Posted in Blog, Tips and Applications | Tagged , | Comments Off on BluePippin “Mid-Range” Size Selection: 18-27 kb

BluePippin:Collecting all DNA “Greater Than” 1kb

We have received several requests to use the Pippin to collect all remaining DNA above a programmed base pair value from a sample. Although Pippins currently have the capability to collect all DNA after a run time threshold (using the … more »

Posted in Blog, Tips and Applications | Tagged , | Comments Off on BluePippin:Collecting all DNA “Greater Than” 1kb

New Cassettes Use Internal Standards

We’ve just released new dye-free cassettes for the BluePippin and Pippin Prep that use dye-labeled DNA markers* as internal standards, instead of typical external marker set. In the new product, the labeled markers will come premixed wi th the Ficoll … more »

Posted in Blog, Tips and Applications | Tagged , , , | 2 Comments

“Overflow Detection” definition on the Pippin Prep to be eliminated

| | Pippin Prep users are likely familiar with our “overflow detection” cassette definitions that are found in the “Cassette Type” drop down menu of the Protocol Editor. We use these definitions because Pippin cassettes exhibit a phenomenon where the … more »

Posted in Blog, Tips and Applications | Tagged , | Comments Off on “Overflow Detection” definition on the Pippin Prep to be eliminated

ChIP-Seq sample prep tip – Interpreting the apparent presence of a secondary band in size-selected DNA

ChIP-Seq analysis is used to study the control of gene expression by identifying DNA regions that are associated  with chromatin at the time of cellular analysis. The ChIP-Seq procedure typically involves cross-linking DNA to the associated chromatin proteins, sonication to … more »

Posted in Blog, Tips and Applications | Tagged , , , , , , , , | Comments Off on ChIP-Seq sample prep tip – Interpreting the apparent presence of a secondary band in size-selected DNA