Tag Archives: SageELF

Poster: Gauging Repeat Expansion Size Quickly for Clinical Use

Patients with hereditary ALS-FTLD (Lou Gehrig’s disease, marked by frontotemporal lobar degeneration) typically have a hexanucleotide repeat expansion in C9orf72. The size of that repeat expansion can be indicative of age of onset, severity of symptoms, and more, so it’s … more »

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PacBio Users: Size Selection Is Essential for Generating Excellent Results

The Sage Science team was delighted to attend and co-sponsor PacBio’s annual East Coast user group meeting in Baltimore last week, particularly since there was a half-day session devoted to our favorite subject: sample prep. There were plenty of customer … more »

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Long-Read Isoform Sequencing Reveals a Hidden Complexity of the Transcriptional Landscape of Herpes Simplex Virus Type 1

June 2017 Authors: Dóra Tombácz, Zsolt Csabai, Attila Szűcs, Zsolt Balázs, et al. Info: Scientists from Hungary and California conducted isoform sequencing with PacBio to analyze the transcriptome of herpes simplex virus type 1. They discovered dozens of previously unidentified … more »

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Comprehensive comparison of Pacific Biosciences and Oxford Nanopore Technologies and their applications to transcriptome analysis

June 2017 Authors: Jason Weirather, Mariateresa de Cesare, Yunhao Wang, Paolo Piazza, Vittorio Sebastiano, Xiu-Jie Wang, David Buck, Kin Fai Au Info: In this F1000 publication, scientists describe an evaluation of long-read sequencing technologies, finding that PacBio provides better data … more »

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Targeted capture and sequencing of gene-sized DNA molecules

December 2016 Authors: Michael Giolai, Pirita Paajanen, Walter Verweij, Lawrence Percival-Alwyn, et al. Info: Scientists adapted the RenSeq capture method for long-read PacBio sequencing, optimizing the method through improved shearing, size selection, and PCR amplification. They used SageELF extensively to … more »

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Preprints Galore!

We normally wait until papers come out in scientific journals before reporting on them here, but there are so many great preprints featuring Sage Science tools we couldn’t resist pointing them out. (On a side note, the rising numbers of … more »

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At the University of Florida, SageELF Delivers Better Sizing for ddRAD-seq and Long-Read Pipelines

Clients of the NextGen DNA Sequencing core at the University of Florida in Gainesville rely on Scientific Director David Moraga Amador to find and validate the best technologies for their projects. In addition to bringing in the best sequencers, that … more »

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At the University of Delaware, Early Adopter Genomics Core Spots New Science Trends

The genomics core facility at the University of Delaware has set itself apart from other service providers by being among the first to adopt new sequencing technologies. The strategy has been a success: today, the facility serves customers around the … more »

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NCSU Scientist Incorporates Genomics for Better Blueberry Resources

Hamid Ashrafi is working to breed higher-quality blueberries that are amenable to mechanical harvest, larger in size, tastier, and have a longer shelf life. As an assistant professor at North Carolina State University, Ashrafi is bringing genomic tools to a … more »

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SageELF Offers Unique Advantages for Long-Range Sequencing and CNV Analysis

We released our SageELF instrument two years ago, and seeing how scientists have adopted it for various NGS pipelines has been a wonderful journey. If you haven’t noticed these great uses, we’ll get you up to speed with this quick … more »

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