For Illumina and short-read sequencing applications, BluePippin users typically use either high-resolution 3% agarose gel cassettes and/or slightly lower resolution 2% agarose gel cassettes. The 3% cassettes are great for “threading the needle” when separating microRNA libraries from nearby unincorporated adapter dimers. The 2% cassettes provide a wider range of collection capabilities (100-600bp vs 100-250bp) with sufficient resolution for most other short-read methods.
However, we have received some customer requests for higher accuracy size selection above 250bp. So we’ve assembled a 2.5% agarose cassette to split the difference in resolution. With this cassette, we can provide better size selection accuracy and reproducibility for size selections across the same 100-600bp range.
If you feel that you can benefit from this, order part #BDF2510. These do require about a 15-25% longer run time than the 2% cassettes.
Check out the performance specification comparison between the two products below:
2.5% Agarose Gel Cassettes (BDF2510)
2% Agarose Gel Cassettes (BDF2010)
