Targeted capture and sequencing of gene-sized DNA molecules

December 2016

Michael Giolai, Pirita Paajanen, Walter Verweij, Lawrence Percival-Alwyn, et al.

Scientists adapted the RenSeq capture method for long-read PacBio sequencing, optimizing the method through improved shearing, size selection, and PCR amplification. They used SageELF extensively to remove small fragments from libraries so the sequencer would generate the longest reads possible.

BioTechniques 61:315-322
doi 10.2144/000114484

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