Dóra Tombácz, Zsolt Csabai, Attila Szűcs, Zsolt Balázs, et al.
Scientists from Hungary and California conducted isoform sequencing with PacBio to analyze the transcriptome of herpes simplex virus type 1. They discovered dozens of previously unidentified protein-coding genes and non-coding RNAs and found that transcription may facilitate crosstalk between distant genes. They used both BluePippin and SageELF to remove shorter fragments and focus libraries on the longest molecules.
Front Microbiol. 2017; 8: 1079.