We are pleased to introduce the MegaPulse™ (MGP0200), a new pulsed-field electrophoresis power supply that supports both Field-Inversion Gel Electrophoresis (FIGE) and Zero-Integrated-Field Electrophoresis (ZIFE) while running one or two standard agarose midi gel boxes simultaneously. With seven pre-validated protocols covering 0.5 kb to 1.6 Mb, the MegaPulse provides complete HMW DNA analysis in a single, compact instrument.
In a typical FIGE workflow, DNA fragments above ~450 kb compress into an unresolved band. ZIFE overcomes this by simultaneously switching the field magnitude, direction, and pulse time so that the net time-integrated field over each pulse cycle is near zero. Under these conditions, very large DNA molecules are resolved on the basis of the time required to reorient in response to field changes — extending practical resolution to 1.6 Mb on a standard agarose slab gel.
The MegaPulse includes seven pre-validated protocols covering 0.5 kb to 1.6 Mb across both FIGE and ZIFE modes. Users who want to go beyond the pre-sets can build and save custom waveforms using the Advanced Programming interface.
The MegaPulse is controlled by any Windows PC running our MegaPulse software. For labs that want a turnkey setup, we offer an optional mini PC tablet with touchscreen with the MegaPulse software pre-loaded. The instrument can also drive one or two Sage Pulse Midi Gel Boxes (PGB1000) simultaneously using the same protocol.
The MegaPulse is well-suited for HMW genomic DNA QC ahead of long-read sequencing library prep and any pulsed-field electrophoresis application in the 0.5 kb–1.6 Mb range.
To order, visit our product page to view available configurations and bundles. Contact us at info@sagescience.com or visit sagescience.com for more information and to download the operations manual.