Tag Archives: Pippin Prep

Urbanization shapes the demographic history of a native rodent (the white-footed mouse, Peromyscus leucopus) in New York City

April 2016 Authors: Stephen E. Harris, Alexander T. Xue, Diego Alvarado-Serrano, Joel T. Boehm, Tyler Joseph, Michael J. Hickerson, Jason Munshi-South Info: Researchers at the City University of New York studied populations of white-footed mice in New York City and … more »

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New hyRAD Method Expands Use of RAD-seq to Degraded Samples

Since RAD-seq was first developed, we’ve seen a number of new versions and approaches from an enthusiastic scientific community. The latest was recently published in PLoS One and demonstrates a RAD-based method suitable for analyzing degraded DNA, an essential step … more »

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Hybridization Capture Using RAD Probes (hyRAD), a New Tool for Performing Genomic Analyses on Collection Specimens

March 2016 Authors: Tomasz Suchan, Camille Pitteloud, Nadezhda S. Gerasimova, Anna Kostikova, Sarah Schmid, Nils Arrigo, Mila Pajkovic, Michał Ronikier, and Nadir Alvarez Info: Scientists present a new protocol based on the RAD-seq concept that works more effectively for samples … more »

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Identification of Human Islet Amyloid Polypeptide as a BACE2 Substrate

February 2016 Authors: Ingrid C. Rulifson, Ping Cao, Li Miao, David Kopecky, Linda Huang, Ryan D. White, Kim Samayoa, Jonitha Gardner, Xiaosu Wu, Kui Chen, Trace Tsuruda, Oliver Homann, Helene Baribault, Harvey Yamane, Tim Carlson, Jed Wiltzius, Yang Li Info: … more »

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A comprehensive benchmarking study of protocols and sequencing platforms for 16S rRNA community profiling

January 2016 Authors: Rosalinda D’Amore, Umer Zeeshan Ijaz, Melanie Schirmer, John G. Kenny, Richard Gregory, Alistair C. Darby, Migun Shakya, Mircea Podar, Christopher Quince and Neil Hall Info: Scientists used synthetic metagenomic communities to evaluate the performance of different sequencing … more »

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Harnessing the power of RADseq for ecological and evolutionary genomics

January 2016 Authors: Kimberly R. Andrews, Jeffrey M. Good, Michael R. Miller, Gordon Luikart and Paul A. Hohenlohe Info: In this protocol review, scientists examine several approaches to restriction site-associated DNA sequencing and present their findings on how to choose … more »

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For MicroRNA Discovery, Pippin Prep Delivers Highest Yield and Specificity

In a highly accessed paper in BMC Medical Genomics, scientists from McGill University and EMBL tested several steps to find the most robust pipeline for discovering small non-coding RNAs (sncRNAs) that might be useful as biomarkers. As part of this … more »

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Next generation sequencing to identify novel genetic variants causative of autosomal dominant familial hypercholesterolemia associated with increased risk of coronary heart disease

July 2015 Authors: Faisal A. Al-Allaf, Mohammad Athar, Zainularifeen Abduljaleel, Mohiuddin M. Taher, Wajahatullah Khan, Faisal A. Ba-hammam, Hala Abalkhail, Abdullah Alashwal Info: Scientists from Saudi Arabia published a paper in Gene demonstrating the use of targeted sequencing to detect … more »

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Isolation via next-generation sequencing of microsatellites from the Tasmanian macroalgae Lessonia corrugata (Lessoniaceae)

July 2015 Authors: Halley M. S. Durrant, Christopher P. Burridge, and Michael G. Gardner Info: Scientists from Australia developed microsatellite markers for this macroalgae, an important component of the local marine ecosystem, that will allow for future population genetic studies … more »

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A genomic selection component analysis characterizes migration-selection balance

July 2015 Authors: Patrick J. Monnahan, Jack Colicchio, and John K. Kelly Info: In the journal Evolution, scientists from the University of Kansas report the population study of a plant species in novel habitat, which allowed them to carefully monitor … more »

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