A comprehensive benchmarking study of protocols and sequencing platforms for 16S rRNA community profiling

January 2016

Rosalinda D’Amore, Umer Zeeshan Ijaz, Melanie Schirmer, John G. Kenny, Richard Gregory, Alistair C. Darby, Migun Shakya, Mircea Podar, Christopher Quince and Neil Hall

Scientists used synthetic metagenomic communities to evaluate the performance of different sequencing and analysis pipelines. They assessed platforms including MiSeq, the PacBio RS II, 454 GS-FLX, and Ion Torrent, and found that sample preparation can alter data interpretation. They used Pippin Prep for sizing NGS libraries prior to sequencing.

D’Amore et al. BMC Genomics (2016) 17:55
DOI 10.1186/s12864-015-2194-9

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