A comprehensive benchmarking study of protocols and sequencing platforms for 16S rRNA community profiling

January 2016

Authors:
Rosalinda D’Amore, Umer Zeeshan Ijaz, Melanie Schirmer, John G. Kenny, Richard Gregory, Alistair C. Darby, Migun Shakya, Mircea Podar, Christopher Quince and Neil Hall

Info:
Scientists used synthetic metagenomic communities to evaluate the performance of different sequencing and analysis pipelines. They assessed platforms including MiSeq, the PacBio RS II, 454 GS-FLX, and Ion Torrent, and found that sample preparation can alter data interpretation. They used Pippin Prep for sizing NGS libraries prior to sequencing.

Citation:
D’Amore et al. BMC Genomics (2016) 17:55
DOI 10.1186/s12864-015-2194-9

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