Tag Archives: Pippin Prep

Skin Microbiome Imbalance in Patients with STAT1/STAT3 Defects Impairs Innate Host Defense Responses

Posted on June 12, 2013 by Alex

Authors: Sanne P. Smeekens, c Curtis Huttenhower, Anca Riza, Frank L. van de Veerdonk, Patrick L.J.M. Zeeuwen, Joost Schalkwijk, Jos W.M. van der Meer, Ramnik J. Xavier, Mihai G. Netea, Dirk Gevers Info: Genomic DNA was subjected to 16S PCR … more »

Posted in Citation | Tagged , , , | Comments Off on Skin Microbiome Imbalance in Patients with STAT1/STAT3 Defects Impairs Innate Host Defense Responses

A Filtering Method to Generate High Quality Short Reads Using Illumina Paired-End Technology

Posted on June 2, 2013 by Alex

June 2013 Authors: A. Murat Eren, Joseph H. Vineis., Hilary G. Morrison, Mitchell L. Sogin Info: The authors present a method of library preparation for overlapping paired-end sequencing on the Illumina platform, and improved analysis software, to reduce the error … more »

Posted in Citation | Tagged , , , , | Comments Off on A Filtering Method to Generate High Quality Short Reads Using Illumina Paired-End Technology

New Prostate Cancer Research Indicates Rapid Accumulation of DNA Mutations

Posted on May 31, 2013 by Alex

Researchers studying the behavior of prostate cancer have shed light on how the cancer develops mutations so rapidly. In contrast to other cancers that seem to progress by adding mutations one at a time, the prostate cancer samples that were … more »

Posted in Blog | Tagged , | Comments Off on New Prostate Cancer Research Indicates Rapid Accumulation of DNA Mutations

Deciphering the Growth Behaviour of Mycobacterium africanum

Posted on May 24, 2013 by Alex

May 2013 Authors: Florian Gehre, Jacob Otu, Kathryn DeRiemer, Paola Florez de Sessions, Martin L. Hibberd, Wim Mulders, Tumani Corrah, Bouke C. de Jong, Martin Antonio Info: The Pippin Prep was used to size select libraries prepared with the New … more »

Posted in Citation | Tagged , , , | Comments Off on Deciphering the Growth Behaviour of Mycobacterium africanum

Multiplex target capture with double-stranded DNA probes

Posted on May 23, 2013 by Alex

May 2013 Authors: Peidong Shen, Wenyi Wang, Aung-Kyaw Chi, Yu Fan, Ronald W Davis, Curt Scharfe Info: The authors describe a new method for targeted NGS using double-stranded probes rather than single-stranded ones, improving sensitivity for variant detection, and at … more »

Posted in Citation | Tagged , , | Comments Off on Multiplex target capture with double-stranded DNA probes

RESTseq – Efficient Benchtop Population Genomics with RESTriction Fragment SEQuencing

Posted on May 10, 2013 by Alex

May 2013 Authors: Eckart Stolle1, Robin F. A. Moritz Info: The authors present a new method for SNP analysis in population genomics. The Pippin Prep was used to size select DNA for the Ion Torrent PGM platform . Citation: PLoS … more »

Posted in Citation | Tagged , | Comments Off on RESTseq – Efficient Benchtop Population Genomics with RESTriction Fragment SEQuencing

FOXO1 regulates expression of a microRNA cluster on X chromosome

Posted on May 2, 2013 by Alex

May 2013 Authors: Ruchi Singhal, Jonathan E. Bard, Norma J. Nowak, Michael J.Buck, and Eugene S. Kandel Info: The Pippin Prep was used in size selection with the Illumina TruSeq Small RNA sequencing protocol. Citation: AGING 5(5): 347-356 http://www.impactaging.com/papers/v5/n5/full/100558.html

Posted in Citation | Tagged , , , | Comments Off on FOXO1 regulates expression of a microRNA cluster on X chromosome

Shining a Light on Dark Sequencing: Characterising Errors in Ion Torrent PGM Data

Posted on April 23, 2013 by Alex

April 2013 Authors: Lauren M. Bragg, Glenn Stone, Margaret K. Butler, Philip Hugenholtz, Gene W. Tyson Info: The authors use the Pippin Prep as an alternative to gel purification for isolating PCR fragments. The authors note that manual gel purification … more »

Posted in Citation | Tagged , , , , | Comments Off on Shining a Light on Dark Sequencing: Characterising Errors in Ion Torrent PGM Data

Transcriptome Analysis of Thapsia laciniata Rouy Provides Insights into Terpenoid Biosynthesis and Diversity in Apiaceae

Posted on April 10, 2013 by Alex

April 2013 Authors: Damian Paul Drew, Bjørn Dueholm, Corinna Weitzel, Ye Zhang, Christoph W. Sensen, and Henrik Toft Simonsen Info: The Pippin Prep was used to size-select Illumina RNA-seq libraries. Citation: Int. J. Mol. Sci. 2013, 14, 9080-9098 http://dx.doi.org/10.3390/ijms14059080

Posted in Citation | Tagged , , , | Comments Off on Transcriptome Analysis of Thapsia laciniata Rouy Provides Insights into Terpenoid Biosynthesis and Diversity in Apiaceae

NHGRI Scientists Find Transgenic DNA Has More Rearrangements than Expected

Posted on April 8, 2013 by Alex

Amanda DuBose didn’t set out to come up with a new technical solution to characterizing transgenic DNA in animal models. A postdoctoral fellow in Francis Collins’ lab at the National Human Genome Research Institute, DuBose was using mice to study … more »

Posted in Blog | Tagged , | Comments Off on NHGRI Scientists Find Transgenic DNA Has More Rearrangements than Expected