Shining a Light on Dark Sequencing: Characterising Errors in Ion Torrent PGM Data

April 2013

Lauren M. Bragg, Glenn Stone, Margaret K. Butler, Philip Hugenholtz, Gene W. Tyson

The authors use the Pippin Prep as an alternative to gel purification for isolating PCR fragments. The authors note that manual gel purification can lead to underrepresentation of low G+C% sequences.

PLoS Comput Biol 9(4):e1003031

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