Shining a Light on Dark Sequencing: Characterising Errors in Ion Torrent PGM Data

April 2013

Authors:
Lauren M. Bragg, Glenn Stone, Margaret K. Butler, Philip Hugenholtz, Gene W. Tyson

Info:
The authors use the Pippin Prep as an alternative to gel purification for isolating PCR fragments. The authors note that manual gel purification can lead to underrepresentation of low G+C% sequences.

Citation:
PLoS Comput Biol 9(4):e1003031

http://dx.doi.org/10.1371/journal.pcbi.1003031

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