Multiplex target capture with double-stranded DNA probes

May 2013

Peidong Shen, Wenyi Wang, Aung-Kyaw Chi, Yu Fan, Ronald W Davis, Curt Scharfe

The authors describe a new method for targeted NGS using double-stranded probes rather than single-stranded ones, improving sensitivity for variant detection, and at a lower cost. The probes consist of single-stranded complimentary long padlock probes (CLPPs), each of which selectively captures one strand of a genomic target through circularization. The Pippin Prep was used to size select libraries for the Illumina MiSeq platform.

Genome Medicine 2013, 5:50

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