Peidong Shen, Wenyi Wang, Aung-Kyaw Chi, Yu Fan, Ronald W Davis, Curt Scharfe
The authors describe a new method for targeted NGS using double-stranded probes rather than single-stranded ones, improving sensitivity for variant detection, and at a lower cost. The probes consist of single-stranded complimentary long padlock probes (CLPPs), each of which selectively captures one strand of a genomic target through circularization. The Pippin Prep was used to size select libraries for the Illumina MiSeq platform.
Genome Medicine 2013, 5:50