Heat selection enables highly scalable methylome profiling in cell-free DNA for noninvasive monitoring of cancer patients

September 2022

Elsie Cheruba, Ramya Viswanathan, Pui-Mun Wong, Howard John Womersley,
Shuting Han, Brenda Tay, Yiting Lau, Anna Gan, Polly S. Y. Poon, Anders Skanderup,
Sarah B. Ng, Aik Yong Chok, Dawn Qingqing Chong, Iain Beehuat Tan, Lih Feng Cheow

The authors outline a method (Heatrich-BS) for enriching GC-rich regions (CpG islands) in cell free DNA by heating the sequencing libraries prior to performing bisulfite sequencing. Since methylation profiles are key biomarkers for many cancers, this method provides a low cost and scalable method for clinical assays. The authors also provide an algorithm for monitoring response to colorectal cancer treatment in patients.

BluePippin was used size select the libraries prior to sequencing.

Author Affiliations:
Department of Biomedical Engineering, National University of Singapore
Institute for Health Innovation and Technology, National University of Singapore
Genome Institute of Singapore, Agency for Science, Technology, and Research, Singapore
Division of Medical Oncology, National Cancer Centre Singapore,
Department of Colorectal Surgery, Singapore General Hospital, Singapore
Medical School, National University of Singapore, Singapore

Science Advances
DOI: 10.1126/sciadv.abn4030

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