A method to simultaneously construct up to 12 differently sized Illumina Nextera long mate pair libraries with reduced DNA input, time, and cost

July 2015

Darren Heavens, Gonzalo Garcia Accinelli, Bernardo Clavijo, and Matthew Derek Clark

Scientists from The Genome Analysis Centre report a protocol using the SageELF to improve library preparation for long mate-pair sequencing. Using a Nextera kit, they show that generating 12 fractions with SageELF saves money and time while requiring less DNA input compared to other gel-based sizing options.

BioTechniques 59:42-45 (July 2015)
DOI: 10.2144/000114310

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