Tag Archives: Pippin Prep

Genetic mapping of horizontal stripes in Lake Victoria cichlid fishes: benefits and pitfalls of using RAD markers for dense linkage mapping

Posted on October 2, 2014 by Alex

August 2014 Authors: Frederico Henning, Hyuk Je Lee, Paolo Franchini and Axel Meyer Info: Scientists from the University of Konstanz in Germany use double-digest RADseq to determine the genetic basis for horizontal stripes seen on cichlid fishes. Sequencing was performed … more »

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Illumina Workflow: Pippin for Massively Parallel Genotyping

Posted on October 1, 2014 by Alex

With so many Sage customers using their Pippin instruments in an Illumina sequencer pipeline, we’re taking a look at various applications enabled by the Sage + Illumina combination. Today we check out double-digest RADseq, which could not work without precise … more »

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In Nature Biotech Paper, ABRF Group Reports Cross-Platform RNA-seq Findings

Posted on September 17, 2014 by Alex

If there’s a group you can count on to do the detailed work of putting instruments through their paces to help scientists perform better science, it’s the Association of Biomolecular Resource Facilities. In a new Nature Biotechnology paper, the next-gen … more »

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Illumina Workflow: Pippin for ChIP-seq

Posted on September 16, 2014 by Alex

As we continue our blog series on applications that are frequently used with Pippin size selection and Illumina sequencing, we move on to ChIP-seq. One of the most popular capabilities enabled by next-gen sequencing, ChIP-seq (or chromatin immunoprecipitation sequencing) is … more »

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1,000th Pippin Customer: Cave Scientist Extraordinaire

Posted on September 2, 2014 by Alex

At the University of Akron, geology and biology professor Hazel Barton shows her students the ropes. Literally. A veteran caver, Barton combined her passion for exploring some of the most remote locations in the world with her scientific interest in … more »

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Multisubstrate Isotope Labeling and Metagenomic Analysis of Active Soil Bacterial Communities

Posted on July 10, 2014 by Alex

July 2014 Authors: Y. Verastegui, J. Cheng, K. Engel, D. Kolczynski, S. Mortimer, J. Lavigne, J. Montalibet, T. Romantsov, M. Hall, B. J. McConkey, D. R. Rose, J. J. Tomashek, B. R. Scott, T. C. Charles, J. D. Neufeld Info: … more »

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Single-Step Selection of Bivalent Aptamers Validated by Comparison with SELEX Using High-Throughput Sequencing

Posted on June 10, 2014 by Alex

June 2014 Authors: Robert Wilson, Christian Bourne, Roy R. Chaudhuri, Richard Gregory, John Kenny, Andrew Cossins Info: This study, conducted by researchers at the University of Liverpool, aimed at accelerating the identification of nucleic acid aptamers by finding ways to … more »

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Improved Multiple Displacement Amplification (iMDA) and Ultraclean Reagents

Posted on June 5, 2014 by Alex

June 2014 Authors: S Timothy Motley, John M Picuri, Chris D Crowder, Jeremiah J Minich, Steven A Hofstadler and Mark W Eshoo Info: In this methods paper, researchers from Ibis Biosciences report a superior whole-genome amplification protocol that can be … more »

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Methylation-capture and Next-Generation Sequencing of free circulating DNA from human plasma

Posted on June 3, 2014 by Alex

June 2014 Authors: Kristina Warton, Vita Lin, Tina Navin, Nicola J Armstrong, Warren Kaplan, Kevin Ying, Brian Gloss, Helena Mangs, Shalima S Nair, Neville F Hacker, Robert L Sutherland, Susan J Clark and Goli Samimi1 Info: This study from the … more »

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SimRAD: a R package for simulation-based prediction of the number of loci expected in RADseq and similar genotyping by sequencing approaches

Posted on May 15, 2014 by Alex

May 2014 (epub ahead of print) Authors: Olivier Lepais and Jason T Weir Info: This article from Molecular Ecology Resources describes a new software tool designed to help scientists optimize their double-digest RADseq (ddRADseq) experiments by accurately estimating the number … more »

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