Multisubstrate Isotope Labeling and Metagenomic Analysis of Active Soil Bacterial Communities

July 2014

Y. Verastegui, J. Cheng, K. Engel, D. Kolczynski, S. Mortimer, J. Lavigne, J. Montalibet, T. Romantsov, M. Hall, B. J. McConkey, D. R. Rose, J. J. Tomashek, B. R. Scott, T. C. Charles, J. D. Neufeld

In this study, researchers from the University of Waterloo and Iogen Corporation used functional metagenomics and DNA stable-isotope probing to characterize bacterial communities in soil. They also focused on glycoside hydrolase genes for their value in industrial use. Sequencing was done with Illumina HiSeq and size selection was performed with Pippin Prep to choose fragments between 400 bp and 800 bp.

mBio 5(4): doi:10.1128/mBio.01157-14

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