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Tag Archives: Pippin Prep
Assessing the utility of whole genome amplified DNA for next-generation molecular ecology
February 2015 Authors: Christopher Blair, C. Ryan Campbell, and Anne D. Yoder Info: Scientists at Duke University explored whether whole-genome amplification leads to sequence bias by performing a ddRAD-seq study of grey mouse lemurs using multiple displacement amplification. They conclude … more »
Posted in Citation
Tagged ddRADseq, Pippin Prep
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Novel Approach to Quieting MYC Shows Potential for Cancer Treatment
It’s hard to have an understated response to seeing our products used in the field when the papers coming out of the work are as extraordinary as this new study from Rick Young at the Whitehead Institute and his collaborators … more »
Posted in Blog
Tagged Pippin Prep
Comments Off on Novel Approach to Quieting MYC Shows Potential for Cancer Treatment
ASHG 2014: See You in San Diego!
We can’t wait for the annual conference for the American Society of Human Genetics in San Diego this weekend! Five days of back-to-back scientific sessions, 6,500 attendees, countless parties — it’s a great opportunity to geek out on genomics. While … more »
Posted in Blog
Tagged ASHG, BluePippin, PacBio, Pippin Prep, SageELF
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Illumina Workflow: Pippin for Improved Assembly Accuracy
As we’ve seen throughout this blog series, Sage customers are conducting all sorts of great experiments pairing their Pippin size selection instruments with Illumina sequencers. Today we look at the final topic in this thread: boosting assembly accuracy with precise … more »
Posted in Blog
Tagged assembly, BluePippin, ILMN, Pippin Prep, PippinHT, SageELF
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Placental miR-1301 is dysregulated in early-onset preeclampsia and inversely correlated with maternal circulating leptin
September 2014 Authors: M.S. Weedon-Fekjær, Y. Sheng, M. Sugulle, G.M. Johnsen, F. Herse, C.W. Redman, R. Lyle, R. Dechend, A.C. Staff Info: Scientists from Norway, the UK, and Germany used sequenced small RNAs in placenta from patients with and without … more »
Posted in Citation
Tagged miRNA, Pippin Prep
Comments Off on Placental miR-1301 is dysregulated in early-onset preeclampsia and inversely correlated with maternal circulating leptin
Evaluation of a Pyrenophora teres f. teres mapping population reveals multiple independent interactions with a region of barley chromosome 6H
August 2014 Authors: Rachel A. Shjerve, Justin D. Faris, Robert S. Brueggeman, Changhui Yan, Ya Zhu, Vaidehi Koladia, Timothy L. Friesen Info: Researchers in North Dakota studied the genetics of virulence in a fungal pathogen that affects barley. They looked … more »
Posted in Citation
Tagged Ion Torrent, Pippin Prep
Comments Off on Evaluation of a Pyrenophora teres f. teres mapping population reveals multiple independent interactions with a region of barley chromosome 6H
Genetic mapping of horizontal stripes in Lake Victoria cichlid fishes: benefits and pitfalls of using RAD markers for dense linkage mapping
August 2014 Authors: Frederico Henning, Hyuk Je Lee, Paolo Franchini and Axel Meyer Info: Scientists from the University of Konstanz in Germany use double-digest RADseq to determine the genetic basis for horizontal stripes seen on cichlid fishes. Sequencing was performed … more »
Posted in Citation
Tagged ddRADseq, Illumina, Pippin Prep
Comments Off on Genetic mapping of horizontal stripes in Lake Victoria cichlid fishes: benefits and pitfalls of using RAD markers for dense linkage mapping
Illumina Workflow: Pippin for Massively Parallel Genotyping
With so many Sage customers using their Pippin instruments in an Illumina sequencer pipeline, we’re taking a look at various applications enabled by the Sage + Illumina combination. Today we check out double-digest RADseq, which could not work without precise … more »
Posted in Blog
Tagged ddRADseq, Illumina, ILMN, Pippin Prep
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In Nature Biotech Paper, ABRF Group Reports Cross-Platform RNA-seq Findings
If there’s a group you can count on to do the detailed work of putting instruments through their paces to help scientists perform better science, it’s the Association of Biomolecular Resource Facilities. In a new Nature Biotechnology paper, the next-gen … more »
Posted in Blog
Tagged NGS sample prep, Pippin Prep
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Illumina Workflow: Pippin for ChIP-seq
As we continue our blog series on applications that are frequently used with Pippin size selection and Illumina sequencing, we move on to ChIP-seq. One of the most popular capabilities enabled by next-gen sequencing, ChIP-seq (or chromatin immunoprecipitation sequencing) is … more »
Posted in Blog
Tagged ChIP-Seq, ILMN, Pippin Prep
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