Improving microbial fitness in the mammalian gut by in vivo temporal functional metagenomics

March 2015

Stephanie J Yaung, Luxue Deng, Ning Li, Jonathan L Braff, George M Church, Lynn Bry, Harris H Wang, Georg K Gerber

In this Molecular Systems Biology paper, researchers from Harvard and Columbia discuss the use of metagenomic DNA to construct large-scale heterologous expression libraries that are tracked over time in vivo by deep sequencing and computational methods. This is the first demonstration of temporal functional metagenomics using shotgun libraries applied to the in vivo mammalian gut environment. Pippin Prep was used for size selection prior to Sanger sequencing.

Mol Syst Biol. (2015) 11: 788
doi 10.15252/msb.20145866

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