Enhanced Reduced Representation Bisulfite Sequencing for Assessment of DNA Methylation at Base Pair Resolution

February 2015

Francine E. Garrett-Bakelman, Caroline K. Sheridan, Thadeous J. Kacmarczyk, Jennifer Ishii, Doron Betel, Alicia Alonso, Christopher E. Mason, Maria E. Figueroa, and Ari M. Melnick

In this publication in the Journal of Visualized Experiments, scientists at Weill Cornell Medical College and the University of Michigan describe a protocol to map DNA methylation patterns across the genome with next-gen sequencing instead of microarrays. Where input amounts allow, the approach calls for automated DNA size selection using Pippin Prep.

J Vis Exp. 2015; (96): 52246.
DOI: 10.3791/52246

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