-
Recent Posts
- Cost-effective and Flexible Preimplantation Genetic Testing (PGT) Using Adaptive Sampling-based Targeted Nanopore Sequencing (ASTN-seq)
- CFTR haplotype phasing using long-read genome sequencing from ultra-low input DNA
- Integrated analysis of miRNAs and mRNAs in thousands of single cells
- Leveraging the power of long reads for targeted sequencing
- Resolving the 22q11.2 deletion using CTLR-Seq reveals chromosomal rearrangement mechanisms and individual variance in breakpoints
Tags
10x Genomics ABRF agbt Amplicon ASHG ASMS BluePippin ChIP-Seq ddRADseq DNA Sequencing Featured genotyping High Pass HiSeq HLA HLS HLS-CATCH Illumina ILMN Ion Torrent isoseq Mate-pair Sequencing Methods microRNA miRNA miRNA isolation MiSeq ONT oxford nanopore PacBio PAG Paired-end Sequencing PippinHT Pippin Prep Pippin System podcast Protein Mass Spec Protein Size Fractionation proteomics RAD-seq RNA-seq RNA sequencing SageELF SageHLS Tips and ApplicationsRecent Comments
- Alex on And Now, a Word from Our Customer Service Department
- Donna on And Now, a Word from Our Customer Service Department
- Tony Gill on And Now, a Word from Our Customer Service Department
- Alex on And Now, a Word from Our Customer Service Department
- Andrea on And Now, a Word from Our Customer Service Department
Archives
- January 2025
- December 2024
- July 2024
- June 2024
- April 2024
- February 2024
- January 2024
- December 2023
- August 2023
- June 2023
- March 2023
- February 2023
- January 2023
- November 2022
- September 2022
- April 2022
- January 2022
- September 2021
- July 2021
- June 2021
- May 2021
- February 2021
- January 2021
- November 2020
- October 2020
- July 2020
- May 2020
- April 2020
- March 2020
- February 2020
- January 2020
- November 2019
- August 2019
- July 2019
- June 2019
- May 2019
- April 2019
- March 2019
- February 2019
- January 2019
- December 2018
- October 2018
- September 2018
- May 2018
- April 2018
- March 2018
- February 2018
- January 2018
- December 2017
- November 2017
- October 2017
- September 2017
- August 2017
- July 2017
- June 2017
- May 2017
- April 2017
- March 2017
- February 2017
- January 2017
- December 2016
- November 2016
- October 2016
- September 2016
- August 2016
- July 2016
- June 2016
- May 2016
- April 2016
- March 2016
- February 2016
- January 2016
- December 2015
- November 2015
- October 2015
- September 2015
- August 2015
- July 2015
- June 2015
- May 2015
- April 2015
- March 2015
- February 2015
- January 2015
- December 2014
- November 2014
- October 2014
- September 2014
- August 2014
- July 2014
- June 2014
- May 2014
- April 2014
- March 2014
- February 2014
- January 2014
- December 2013
- November 2013
- October 2013
- September 2013
- August 2013
- July 2013
- June 2013
- May 2013
- April 2013
- March 2013
- February 2013
- January 2013
- December 2012
- November 2012
- October 2012
- September 2012
- August 2012
- July 2012
- June 2012
- May 2012
- April 2012
- March 2012
- February 2012
- December 2011
- October 2011
- September 2011
- June 2011
- February 2011
- November 2010
- October 2010
- August 2010
Tag Archives: PacBio
CFTR haplotype phasing using long-read genome sequencing from ultra-low input DNA
January 2025 Authors: Neeru Gandotra, Antariksh Tyagi, Irina Tikhonova, Caroline Storer, Curt Scharfe Abstract: “Purpose: Newborn screening (NBS) identifies rare diseases that result from the recessive inheritance of pathogenic variants in both copies of a gene. Long-read genome sequencing (LRS) … more »
“Range+T “ for Tight Sizing of HMW Libraries
Range+T Size Selection Note: Range + T is available for all PippinHT instruments. However, only BluePippins with serial numbers 2700 and above will run the method. Sage’s DNA size selection technology has been used to help improve PacBio sequencing, particularly … more »
Posted in Blog
Tagged BluePippin, Diagenode, HiFi, Megaruptor, PacBio, PippinHT, Range+T
Comments Off on “Range+T “ for Tight Sizing of HMW Libraries
Utility of long-read sequencing for All of Us
January 2023 Authors: M. Mahmoud, Y. Huang, K. Garimella, P. A. Audano, W. Wan, N. Prasad, R. E. Handsaker, S. Hall, A. Pionzio, M. C. Schatz, M. E. Talkowski, E. E. Eichler, S. E. Levy, F. J. Sedlazeck Abstract: “The … more »
Posted in Citation
Tagged HiFi, PacBio, PippinHT, whole genome sequencing
Comments Off on Utility of long-read sequencing for All of Us
High-quality Arabidopsis thaliana Genome Assembly with Nanopore and HiFi Long Reads
September 2021 Authors: Bo Wang, Xiaofei Yang, Yanyan Jia, Yu Xu, Peng Jia, Ningxin Dang, Songbo Wang, Tun Xu, Xixi Zhao, Shenghan Gao, Quanbin Dong, Kai Ye Info: The authors report on a high quality genome assembly of the model … more »
Posted in Citation
Tagged BluePippin, HiFi, HLS, oxford nanopore, PacBio
Comments Off on High-quality Arabidopsis thaliana Genome Assembly with Nanopore and HiFi Long Reads
High-molecular weight DNA extraction, clean-up and size selection for long-read sequencing
July 2021 Authors: Ashley Jones ,Cynthia Torkel,David Stanley,Jamila Nasim,Justin Borevitz,Benjamin Schwessinger Info: The authors provide a protocol for preparing high molecular weight DNA sequencing libraries with a particular focus on difficult plant and fungal tissues, and also can be used … more »
Posted in Citation
Tagged oxford nanopore, PacBio, PippinHT
Comments Off on High-molecular weight DNA extraction, clean-up and size selection for long-read sequencing
Comparison of long-read sequencing technologies in interrogating bacteria and fly genomes
June 2021 Authors: Eric S Tvedte, Mark Gasser, Benjamin C Sparklin, Jane Michalski, Carl E Hjelmen, J Spencer Johnston, Xuechu Zhao, Robin Bromley, Luke J Tallon, Lisa Sadzewicz, David A Rasko, Julie C Dunning Hotopp Info: This side-by-side comparison of … more »
Posted in Citation
Tagged BluePippin, oxford nanopore, PacBio
Comments Off on Comparison of long-read sequencing technologies in interrogating bacteria and fly genomes
CRISPR–Cas9/long-read sequencing approach to identify cryptic mutations in BRCA1 and other tumour suppressor genes
October 2020 Authors: Tom Walsh, Silvia Casadei, Katherine M Munson, Mary Eng, Jessica B Mandell, Suleyman Gulsuner, Mary-Claire King Info: In this short report, researchers studying young-onset breast cancer used the SageHLS and HLS-CATCH method to purify high molecular weight … more »
A long-read RNA-seq approach to identify novel transcripts of very large genes
July 2020 Authors: Prech Uapinyoying, Jeremy Goecks, Susan M. Knoblach, Karuna Panchapakesan, Carsten G. Bonnemann, Terence A. Partridge, Jyoti K. Jaiswal, and Eric P. Hoffman Info: Scientists researching muscular disorders present a method for studying gene expression of large RNA … more »
Posted in Citation
Tagged BluePippin, iso-seq, PacBio
Comments Off on A long-read RNA-seq approach to identify novel transcripts of very large genes
Fully phased sequence of a diploid human genome determined de novo from the DNA of a single individual
July 2020 Authors: Ilya Soifer , Nicole L. Fong, Nelda Yi, Andrea T. Ireland, Irene Lam, Matthew Sooknah, Jonathan S. Paw, Paul Peluso, Gregory T. Concepcion, David Rank, Alex R. Hastie, 5 Vladimir Jojic, J. Graham Ruby, David Botstein, Margaret … more »
Posted in Citation
Tagged BluePippin, High Pass, PacBio
Comments Off on Fully phased sequence of a diploid human genome determined de novo from the DNA of a single individual
Highly accurate long-read HiFi sequencing data for five complex genomes
May 2020 Authors: Ting Hon, Kristin Mars, Greg Young, Yu-Chih Tsai, Joseph W. Karalius, Jane M. Landolin, Nicholas Maurer, David Kudrna, Michael A. Hardigan, Cynthia C. Steiner, Steven J. Knapp, Doreen Ware, Beth Shapiro, Paul Peluso, David R. Rank Info: … more »
Posted in Citation
Tagged BluePippin, HiFi, PacBio, SageELF
Comments Off on Highly accurate long-read HiFi sequencing data for five complex genomes