High-molecular weight DNA extraction, clean-up and size selection for long-read sequencing

July 2021

Ashley Jones ,Cynthia Torkel,David Stanley,Jamila Nasim,Justin Borevitz,Benjamin Schwessinger

The authors provide a protocol for preparing high molecular weight DNA sequencing libraries with a particular focus on difficult plant and fungal tissues, and also can be used with animals and microbes. The libraries can be used for PacBio or Oxford Nanopore sequencing, includes clean up and size selection recommendations, in a manner that is economical and scalable.

The PippinHT was used for the DNA size selection recommendations.

Author Affiliations:
Australian National University, Canberra, Australian Capital Territory, Australia

DOI: 10.1371/journal.pone.0253830

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