Comparison of long-read sequencing technologies in interrogating bacteria and fly genomes

June 2021

Eric S Tvedte, Mark Gasser, Benjamin C Sparklin, Jane Michalski, Carl E Hjelmen, J Spencer Johnston, Xuechu Zhao, Robin Bromley, Luke J Tallon, Lisa Sadzewicz, David A Rasko, Julie C Dunning Hotopp

This side-by-side comparison of PacBio and Oxford Nanopore sequencing platforms for the analysis of E.coli and Drosophila assemblies. The authors provide useful guidance for which platform or approach is better suited for different areas of study, as each platform can provide certain advantages. PacBio’s Sequel II CLR, Sequel II HiFi, and RS II were evaluated as was Oxford Nanopore’s Rapid Sequencing and Ligation Sequencing. Hybrid approaches using Illumina sequencing were also evaluated.
BluePippin was used to size select libraries for both platforms.

Author Affiliations:
Institute for Genome Sciences, University of Maryland School of Medicine, Baltimore, MD
Department of Biology, Texas A&M University, College Station, TX
Department of Entomology, Texas A&M University, College Station, TX

G3 Genes|Genomes|Genetics

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