Tag Archives: Illumina

Genome Editing Shows Potential for Shrinking Repeat Expansions

Several diseases, many of them involving neurological symptoms, have been linked to expansions of trinucleotide repeat sequences. These repeat expansion disorders include Fragile X syndrome, Huntington’s disease, and various forms of epilepsy and ataxia. In Fragile X, for example, a … more »

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Quality control of next-generation sequencing data without a reference

May 2014 Authors: Urmi H Trivedi, Timothée Cézard, Stephen Bridgett, Anna Montazam, Jenna Nichols, Mark Blaxter, and Karim Gharbi Info: In this paper published in Frontiers in Genetics, scientists at the University of Edinburgh show that it is possible to … more »

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Highly Specific Contractions of a Single CAG/CTG Trinucleotide Repeat by TALEN in Yeast

April 2014 Authors: Guy-Franck Richard, David Viterbo, Varun Khanna, Valentine Mosbach, Lauriane Castelain, and Bernard Dujon Info: Researchers in France used a TALEN to introduce a double-strand break in a CAG/CCTG repeat in yeast cells, finding that doing so deleted … more »

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The emerging biofuel crop Camelina sativa retains a highly undifferentiated hexaploid genome structure

April 2014 Authors: Sateesh Kagale, Chushin Koh, John Nixon, Venkatesh Bollina, Wayne E. Clarke, Reetu Tuteja, Charles Spillane, Stephen J. Robinson, Matthew G. Links, Carling Clarke, Erin E. Higgins, Terry Huebert, Andrew G. Sharpe & Isobel A.P. Parkin Info: Scientists … more »

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A first genetic map of date palm (Phoenix dactylifera) reveals long-range genome structure conservation in the palms

April 2014 Authors: Lisa S Mathew, Manuel Spannagl, Ameena Al-Malki, Binu George, Maria F Torres, Eman K Al-Dous, Eman K Al-Azwani, Emad Hussein, Sweety Mathew, Klaus FX Mayer, Yasmin Ali Mohamoud, Karsten Suhre and Joel A Malek Info: The first … more »

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DNA methylation is required for the control of stem cell differentiation in the small intestine

March 2014 Authors: Karyn L. Sheaffer, Rinho Kim, Reina Aoki, Ellen N. Elliott, Jonathan Schug, Lukas Burger, Dirk Schubeler, and Klaus H. Kaestner Info: Scientists in Switzerland and Pennsylvania sought to understand the role of methylation in stem cell-directed regeneration … more »

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SASI-Seq: sample assurance Spike-Ins, and highly differentiating 384 barcoding for Illumina sequencing

February 2014 Authors: Michael A Quail, Miriam Smith, David Jackson, Steven Leonard, Thomas Skelly, Harold P Swerdlow, Yong Gu, and Peter Ellis Info: In this paper, scientists from the Wellcome Trust Sanger Institute and Frederick National Laboratory for Cancer Research … more »

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Glutamine methylation in histone H2A is an RNA-polymerase-I-dedicated modification

January 2014 Authors: Peter Tessarz, Helena Santos-Rosa, Sam C. Robson, Kathrine B. Sylvestersen, Christopher J. Nelson1, Michael L. Nielsen and Tony Kouzarides Info: The authors describe a new class of histone modification, methylation of glutamine, occurring on yeast histone H2A. … more »

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Variant calling in low-coverage whole genome sequencing of a Native American population sample

January 2014 Authors: Chris Bizon, Michael Spiegel, Scott A Chasse, Ian R Gizer, Yun Li, Ewa P Malc, Piotr A Mieczkowski, Josh K Sailsbery, Xiaoshu Wang, Cindy L Ehlers and Kirk C Wilhelmsen Info: Scientists from the University of North … more »

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Improved Protocols for Illumina Sequencing

October 2013 Authors: Iraad F. Bronner, Michael A. Quail, Daniel J. Turner, and Harold Swerdlow Info: A comprehensive overview of the state-of-the state of Illumina sequencing, from the Wellcome Trust Sanger Institute and Oxford Nanopore Technologies. The value of narrow … more »

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