SASI-Seq: sample assurance Spike-Ins, and highly differentiating 384 barcoding for Illumina sequencing

February 2014

Michael A Quail, Miriam Smith, David Jackson, Steven Leonard, Thomas Skelly, Harold P Swerdlow, Yong Gu, and Peter Ellis

In this paper, scientists from the Wellcome Trust Sanger Institute and Frederick National Laboratory for Cancer Research describe Sample Assurance Spike-In sequencing, or SASI-Seq. The protocol adds uniquely barcoded amplicons to samples prior to library prep and sequencing; these barcodes allow users to deconvolute samples that get mixed up or to spot cross-contamination issues. They used Pippin Prep to test barcode robustness through the size selection process, finding that barcodes were not affected.

BMC Genomics, 15:110


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