Urmi H Trivedi, Timothée Cézard, Stephen Bridgett, Anna Montazam, Jenna Nichols, Mark Blaxter, and Karim Gharbi
In this paper published in Frontiers in Genetics, scientists at the University of Edinburgh show that it is possible to perform alignment-based quality control on sequence data for organisms that lack a reference genome. They used draft assemblies for organisms representing various genome sizes and found that useful quality control metrics can be inferred from these drafts. The scientists used Illumina sequencing and performed DNA sizing with BluePippin.
Front. Genet. 5:111