Tag Archives: DNA Sequencing

Genetic Basis for the Biosynthesis of the Pharmaceutically Important Class of Epoxyketone Proteasome Inhibitors

Posted on October 7, 2013 by Alex

October 2013 Authors: Michelle Schorn, Judith Zettler, Joseph P. Noel, Pieter C. Dorrestein, Bradley S. Moore, and Leonard Kaysser Info: Collaborators in the U.S. and Germany report on the genetic pathway for the production of epoxyketone proteasome inhibitors, used as … more »

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Quick, sensitive and specific detection and evaluation of quantification of minor variants by high-throughput sequencing

Posted on October 6, 2013 by Alex

October 2013 Authors: Ross Ka-Kit Leung, Zhi Qiang Dong, Fei Sa, Cheong Meng Chong, Si Wan Lei,c Stephen Kwok-Wing Tsui and Simon Ming-Yuen Lee Info: Scientists from the University of Hong Kong and the University of Macau, China, evaluate errors … more »

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Improved Protocols for Illumina Sequencing

Posted on October 1, 2013 by Alex

October 2013 Authors: Iraad F. Bronner, Michael A. Quail, Daniel J. Turner, and Harold Swerdlow Info: A comprehensive overview of the state-of-the state of Illumina sequencing, from the Wellcome Trust Sanger Institute and Oxford Nanopore Technologies. The value of narrow … more »

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Next-Generation Sequence Analysis of Cancer Xenograft Models

Posted on September 1, 2013 by Alex

Authors: Fernando J. Rossello, Richard W. Tothill, Kara Britt, Kieren D. Marini, Jeanette Falzon, David M. Thomas, Craig D. Peacock, Luigi Marchionni, Jason Li, Samara Bennett, Erwin Tantoso, Tracey Brown, Philip Chan, Luciano G. Martelotto, D. Neil Watkins Info: The … more »

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All Conferenced Out: Great Experiences at NGx and BioConference Live

Posted on August 22, 2013 by Alex

We’ve just come off a busy week of conferences! In addition to BioConference Live: Genetics and Genomics, our first virtual scientific meeting, we also attended NGx: Applying Next-Generation Sequencing in Providence, Rhode Island. NGx, one of the best-known next-gen sequencing … more »

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In Sanger Study, BluePippin Contributes to Improved Construction Method for Mate-Pair Libraries

Posted on August 6, 2013 by Alex

Scientists from the Wellcome Trust Sanger Institute recently published “An improved approach to mate-paired library preparation for Illumina sequencing,” a paper that came out in the open access journal Methods in Next Generation Sequencing. The publication, from lead author Naomi … more »

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Comparative genomics of Campylobacter concisus isolates reveals genetic diversity and provides insights into disease association

Posted on August 1, 2013 by Alex

August 2013 Authors: Nandan P Deshpande, Nadeem O Kaakoush, Marc R Wilkins and Hazel M Mitchell Info: Collaborators at the University of New South Wales sequence several strains of Campylobacter concisus from healthy and diseased (Crohn’s disease and gastroenteritis) individuals … more »

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Phylogeographic Origin of Helicobacter pylori Determines Host-Adaptive Responses upon Coculture with Gastric Epithelial Cells

Posted on July 1, 2013 by Alex

July 2013 Authors: Alexander Sheh, Rupesh Chaturvedi, D. Scott Merrell, Pelayo Correa, Keith T. Wilson, James G. Fox Info: A study of gene expression comparing several strains of H. pylori. The BluePippin was used to size-select fragments to construct paired-end … more »

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The new sequencer on the block: comparison of Life Technology’s Proton sequencer to an Illumina HiSeq for whole-exome sequencing

Posted on June 24, 2013 by Alex

June 2013 Authors: Joseph F. Boland, Charles C. Chung, David Roberson, Jason Mitchell, Xijun Zhang, Kate M. Im, Ji He, Stephen J. Chanock, Meredith Yeager, Michael Dean Info: An exome-sequencing study from the NIH comparing Illumina/Nimblegen to Ion Torrent/TargetSeq. Pippin … more »

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From the Trenches: PacBio User Shares Experience with BluePippin

Posted on June 21, 2013 by Alex

As the BluePippin makes its way to more users of the Pacific Biosciences sequencing platform, there is great data emerging on how our high-pass size selection protocol really complements the sequencer. A particularly nice example of this comes from Lex … more »

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