Tag Archives: BluePippin

Highly sensitive detection of mutations in CHO cell recombinant DNA using multi‐parallel single molecule real‐time DNA sequencing

Posted on May 29, 2018 by Alex

June 2018 Authors: Joseph Cartwright, Karin Anderson, Joseph Longworth, Philip Lobb, and David James Info: To improve the quantitative assessment of recombinant DNA in cell lines, University of Sheffield scientists and collaborators developed a PacBio-based method for high-throughput rDNA sequence … more »

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App Note: BluePippin Sizing Yields Longer MinION Reads

Posted on May 17, 2018 by Alex

BluePippin users are generating longer reads from their Oxford Nanopore MinION sequencers, as demonstrated in a new app note from our colleagues at Nippon Genetics. The app note showcases a workflow used by Kazuharu Arakawa, an associate professor in the … more »

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A Whole Genome Assembly of the Horn Fly, Haematobia irritans, and Prediction of Genes with Roles in Metabolism and Sex Determination

Posted on May 15, 2018 by Alex

May 2018 Authors: Kranti Konganti, Felix Guerrero, Faye Schilkey, Peter Ngam, et al. Info: Researchers sequenced, assembled, and annotated the genome of horn fly, a blood-feeding insect that preys on cattle. The 1.14 Gb assembly shows the fly is most … more »

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Intact DNA purified from flow-sorted nuclei unlocks the potential of next-generation genome mapping and assembly in Solanum species

Posted on April 29, 2018 by Alex

April 2018 Authors: Paola Gaiero, Hana Šimková, JanVrána, Federico Santiñaque, et al. Info: Scientists from Wageningen University & Research and other institutions embarked on a project to improve sample prep protocols for obtaining high molecular weight DNA from plant cells … more »

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A Validation Approach of an End-to-End Whole Genome Sequencing Workflow for Source Tracking of Listeria monocytogenes and Salmonella enterica

Posted on April 15, 2018 by Alex

March 2018 Authors: Anne-Catherine Portmann, Coralie Fournier, Johan Gimonet, Catherine Ngom-Bru, et al. Info: Scientists from Nestle Research Center and Nestle Institute of Health Sciences aimed to establish guidelines for whole-genome sequencing used in contamination investigations in the food industry. … more »

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Cost-Effective Microbiome Sequencing with 10x Genomics and Athena Assembler

Posted on March 21, 2018 by Alex

If you missed Ami Bhatt’s talk at AGBT last month, a bioRxiv preprint is a great way to catch up on her team’s impressive work characterizing microbial communities — from the human gut to the sea floor. Bhatt and her … more »

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Small RNA populations revealed by blocking rRNA fragments in Drosophila melanogaster reproductive tissues

Posted on March 12, 2018 by Alex

February 2018 Authors: Emily Fowler, Irina Mohorianu, Damian Smith, Tamas Dalmay, Tracey Chapman Info: Scientists present a method for removing highly abundant rRNAs for improved analysis of small RNAs in RNA interference studies. Results showed increased resolution of sRNAs, even … more »

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The DNA Methylome of the Hyperthermoacidophilic Crenarchaeon Sulfolobus acidocaldarius

Posted on March 12, 2018 by Alex

February 2018 Authors: Mohea Couturier and Ann-Christin Lindås Info: Scientists from Stockholm University investigated the impact of DNA methylation in archaeal genomes by analyzing the complete DNA methylome of S. acidocaldarius using PacBio sequencing. The team used BluePippin to remove … more »

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Draft genome assembly of the Bengalese finch, Lonchura striata domestica, a model for motor skill variability and learning

Posted on March 12, 2018 by Alex

February 2018 Authors: Bradley Colquitt, David Mets, and Michael Brainard Info: Scientists generated a high-quality draft genome and gene annotation for Bengalese finch, which has a more variable song than other finches, making it an important model for sensorimotor learning. … more »

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Genomics of Corynebacterium striatum, an emerging multidrug-resistant pathogen of immunocompromised patients

Posted on February 12, 2018 by Alex

January 2018 Authors: K. Nudel, X. Zhao, S. Basu, X. Dong, et al. Info: Scientists from Harvard Medical School and other institutions used whole genome sequencing and MALDI/TOF to analyze seven C. striatum infections, all resistant to multiple drugs. They … more »

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