Author Archives: Alex

Very Short Mitochondrial DNA Fragments and Heteroplasmy in Human Plasma

Posted on December 9, 2016 by Alex

November 2016 Authors: Ruoyu Zhang, Kiichi Nakahira, Xiaoxian Guo, Augustine M.K. Choi & Zhenglong Gu Info: Scientists at Cornell University studied cell-free mitochondrial DNA found in plasma samples collected from sepsis patients. Along the way, they optimized sample prep protocols … more »

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Preprints Galore!

Posted on December 8, 2016 by Alex

We normally wait until papers come out in scientific journals before reporting on them here, but there are so many great preprints featuring Sage Science tools we couldn’t resist pointing them out. (On a side note, the rising numbers of … more »

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Depletion of tRNA-halves enables effective small RNA sequencing of low-input murine serum samples

Posted on December 8, 2016 by Alex

November 2016 Authors: Alan Van Goethem, Nurten Yigit, Celine Everaert, Myrthala Moreno-Smith, et al. Info: Scientists at Ghent University and Baylor College of Medicine evaluated protocols for depleting abundant RNAs from blood and serum samples to improve the ability to … more »

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Structure of the germline genome of Tetrahymena thermophila and relationship to the massively rearranged somatic genome

Posted on December 8, 2016 by Alex

November 2016 Authors: Eileen P Hamilton, Aurélie Kapusta, Piroska E Huvos, Shelby L Bidwell, et al. Info: A large team of scientists in Asia, Europe, and the U.S. collaborated for this study of Tetrahymena thermophile, for which they produced a complete sequence assembly. They … more »

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Nanopore Sequencers Give Optimal Results with BluePippin Size Selection

Posted on November 1, 2016 by Alex

The Oxford Nanopore team has been speaking recently about their use of our BluePippin automated size selection system for optimizing the read length obtained from nanopore sequencers. For anyone interested in the Oxford platforms who hasn’t seen this information, here’s … more »

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ASHG 2016: Structural Variants, Mega Databases, and the Best-Ever Human Genome

Posted on October 28, 2016 by Alex

The Sage Science team enjoyed our trip across the border to Canada for the annual meeting of the American Society of Human Genetics this month. Vancouver was practically teeming with genome scientists! Many presentations this year featured results generated by … more »

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Get a Glimpse of the New HLS Instrument at ASHG 2016

Posted on October 18, 2016 by Alex

The Sage Science team is heading to Vancouver this week for the annual meeting of the American Society of Human Genetics. ASHG is the biggest genomics meeting we attend each year, and it never fails to deliver on its promise … more »

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Building two indica rice reference genomes with PacBio long-read and Illumina paired-end sequencing data

Posted on October 15, 2016 by Alex

September 2016 Authors: Jianwei Zhang, Ling-Ling Chen, Shuai Sun, et al. Info: In this paper, researchers report generating two reference-quality genome assemblies for Oryza sativa subspecies. They publicly released both assemblies, which were built using PacBio and Illumina sequencing technology. … more »

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Production of the Bioactive Compounds Violacein and Indolmycin Is Conditional in a maeA Mutant of Pseudoalteromonas luteoviolaceaS4054 Lacking the Malic Enzyme

Posted on October 12, 2016 by Alex

September 2016 Authors: Mariane S. Thøgersen, Marina W. Delpin, Jette Melchiorsen, Mogens Kilstrup, Maria Månsson, Boyke Bunk, Cathrin Spröer, Jörg Overmann, Kristian F. Nielsen, and Lone Gram Info: A team of scientists analyzed biological compounds produced by Pseudoalteromonas luteoviolacea, a … more »

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At the University of Florida, SageELF Delivers Better Sizing for ddRAD-seq and Long-Read Pipelines

Posted on October 12, 2016 by Alex

Clients of the NextGen DNA Sequencing core at the University of Florida in Gainesville rely on Scientific Director David Moraga Amador to find and validate the best technologies for their projects. In addition to bringing in the best sequencers, that … more »

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