Author Archives: Alex

The two chromosomes of the mitochondrial genome of a sugarcane cultivar: assembly and recombination analysis using long PacBio reads

August 2016 Authors: Jeremy R. Shearman, Chutima Sonthirod, Chaiwat Naktang, Wirulda Pootakham, Thippawan Yoocha, Duangjai Sangsrakru, Nukoon Jomchai, Somvong Tragoonrung, and Sithichoke Tangphatsornruang Info: Scientists in Thailand used long-read sequencing to assemble the mitochondrial genome of a sugarcane cultivar from … more »

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Poster: Better Cell-Free DNA Analysis with Pippin + Rubicon Genomics

Recently we blogged about the rise of cell-free DNA studies, and how precise size selection is one tool that can help researchers isolate DNA of interest for further analysis (such as sorting out fetal from maternal genetic material). At the AGBT … more »

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Size Selection Has Utility for Cell-Free DNA Studies

The rise of research studies and diagnostic tests looking at cell-free DNA — particularly fetal DNA in a mother’s bloodstream — has happened with astonishing speed. Prenatal genetic testing, for instance, has already supplanted many invasive clinical tests such as … more »

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A draft genome of the brown alga, Cladosiphon okamuranus, S-strain: a platform for future studies of ‘mozuku’ biology

August 2016 Authors: Koki Nishitsuji, Asuka Arimoto, Kenji Iwai, Yusuke Sudo, Kanako Hisata, Manabu Fujie, Nana Arakaki, Tetsuo Kushiro, Teruko Konishi, Chuya Shinzato, Noriyuki Satoh and Eiichi Shoguchi Info: Scientists from Japan sequenced the genome of Cladosiphon okamuranus, an edible … more »

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DSBCapture: in situ capture and sequencing of DNA breaks

August 2016 Authors: Stefanie V Lensing, Giovanni Marsico, Robert Hänsel-Hertsch, Enid Y Lam, David Tannahill & Shankar Balasubramanian Info: Scientists at Cancer Research UK developed and validated a new sequencing-based protocol to spot double-stranded DNA breaks in situ; these regions … more »

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Association of MicroRNAs with Antibody Response to Mycoplasma bovis in Beef Cattle

August 2016 Authors: Eduardo Casas, Guohong Cai, Larry A. Kuehn, Karen B. Register, Tara G. McDaneld, John D. Neill Info: USDA scientists performed a longitudinal study of young cows to identify microRNAs linked to a serum antibody produced after exposure … more »

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Where Illumina Users Gather, Great Science Is on Display

The Sage team is pleased to be sponsoring the slate of upcoming Illumina user group meetings. We’ve attended many of these events over the years, and they’re excellent venues that showcase truly impressive work from the company’s broad customer base. … more »

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Comparative genomics and transcriptomics of Pichia pastoris

August 2016 Authors: Kerry R. Love, Kartik A. Shah, Charles A. Whittaker, Jie Wu, M. Catherine Bartlett, Duanduan Ma, Rachel L. Leeson, Margaret Priest, Jonathan Borowsky, Sarah K. Young and J. Christopher Love Info: To provide a foundation for engineering … more »

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RADcap: sequence capture of dual-digest RADseq libraries with identifiable duplicates and reduced missing data

August 2016 Authors: Hoffberg SL, Kieran TJ, Catchen JM, Devault A, Faircloth BC, Mauricio R, Glenn TC Info: In this methods paper, scientists combine double-digest RAD-seq with capture technology to reduce costs and increase efficiency. The protocol, which was validated … more »

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At the University of Delaware, Early Adopter Genomics Core Spots New Science Trends

The genomics core facility at the University of Delaware has set itself apart from other service providers by being among the first to adopt new sequencing technologies. The strategy has been a success: today, the facility serves customers around the … more »

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