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Tag Archives: Pippin Prep
Towards allele-level human leucocyte antigens genotyping – assessing two next-generation sequencing platforms: Ion Torrent Personal Genome Machine and Illumina MiSeq
June 2015 Authors: J. L. Duke, C. Lind, K. Mackiewicz, D. Ferriola, A. Papazoglou, O. Derbeneva, D. Wallace, and D. S. Monos Info: This publication in the International Journal of Immunogenetics from scientists at the Children’s Hospital of Philadelphia presents … more »
Posted in Citation
Tagged HLA, Illumina, Ion Torrent, Pippin Prep
Comments Off on Towards allele-level human leucocyte antigens genotyping – assessing two next-generation sequencing platforms: Ion Torrent Personal Genome Machine and Illumina MiSeq
At Einstein Med School, ‘Extremely Reproducible’ Pippin Platforms Save Time and Money
In the genetics department at Albert Einstein College of Medicine, Research Assistant Professor Alex Maslov is working to understand structural variants associated with aging and cancer. Using human and mouse cells, he deploys whole genome sequencing to make these links. … more »
Posted in Blog
Tagged Illumina, Ion Torrent, Pippin Prep, PippinHT
Comments Off on At Einstein Med School, ‘Extremely Reproducible’ Pippin Platforms Save Time and Money
Double-digest RAD sequencing using Ion Proton semiconductor platform (ddRADseq-ion) with nonmodel organisms
April 2015 Authors: Hans Recknagel, Arne Jacobs, Pawel Herzyk, Kathryn R Elmer Info: In this Molecular Ecology Resources paper, researchers from the University of Glasgow describe a protocol and adapters they developed for double-digest RAD sequencing on the Ion Torrent … more »
Posted in Citation
Tagged ddRADseq, Ion Torrent, Pippin Prep
Comments Off on Double-digest RAD sequencing using Ion Proton semiconductor platform (ddRADseq-ion) with nonmodel organisms
Improved transposon-based library preparation for the Ion Torrent platform
April 2015 Authors: Tatyana Gorbacheva, Wilber Quispe-Tintaya, Vasily N. Popov, Jan Vijg, and Alexander Y. Maslov Info: In this Biotechniques paper, scientists from the Albert Einstein College of Medicine in New York describe MuPlus, a new transposon-based method for barcoded … more »
Posted in Citation
Tagged Ion Torrent, Pippin Prep
Comments Off on Improved transposon-based library preparation for the Ion Torrent platform
Video Protocol: NGS-based Methylation Mapping with Pippin Prep
We always love a great protocol video, and this one from scientists at Weill Cornell Medical College, published through the Journal of Visualized Experiments, is a keeper. Check it out here: “Enhanced Reduced Representation Bisulfite Sequencing for Assessment of DNA … more »
Posted in Blog
Tagged methylation, Pippin Prep
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PippinHT in the Wild: High-Throughput ChIP-Seq at Whitehead Core Lab
At the Whitehead Institute’s Genome Technology Core, scientists handle a lot of ChIP-seq and RNA-seq projects. To boost capacity in library prep, they recently upgraded from a small fleet of Pippin Prep instruments to the new PippinHT for high-throughput, automated … more »
Posted in Blog
Tagged ChIP-Seq, Pippin Prep, PippinHT
Comments Off on PippinHT in the Wild: High-Throughput ChIP-Seq at Whitehead Core Lab
Discovery of Novel Rhabdoviruses in the Blood of Healthy Individuals from West Africa
March 2015 Authors: Matthew H Stremlau, Kristian G Andersen, Onikepe A Folarin, Jessica N Grove, Ikponmwonsa Odia, Philomena E Ehiane, Omowunmi Omoniwa, Omigie Omoregie, Pan-Pan Jiang, Nathan L Yozwiak, Christian B Matranga, Xiao Yang, Stephen K Gire, Sarah Winnicki, Ridhi … more »
Posted in Citation
Tagged Pippin Prep
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New Structural Variant Analysis Method Uses Targeted Capture and Sequencing of Large-Insert DNA
In a new BMC Genomics paper, scientists from Baylor College of Medicine describe a new method for accurate, affordable interrogation of structural variants across the human genome. We’re delighted to see that automated DNA size selection tools from Sage Science … more »
Posted in Blog
Tagged BluePippin, PacBio, Pippin Prep
Comments Off on New Structural Variant Analysis Method Uses Targeted Capture and Sequencing of Large-Insert DNA
Enhanced Reduced Representation Bisulfite Sequencing for Assessment of DNA Methylation at Base Pair Resolution
February 2015 Authors: Francine E. Garrett-Bakelman, Caroline K. Sheridan, Thadeous J. Kacmarczyk, Jennifer Ishii, Doron Betel, Alicia Alonso, Christopher E. Mason, Maria E. Figueroa, and Ari M. Melnick Info: In this publication in the Journal of Visualized Experiments, scientists at … more »
Posted in Citation
Tagged Pippin Prep
Comments Off on Enhanced Reduced Representation Bisulfite Sequencing for Assessment of DNA Methylation at Base Pair Resolution