Tag Archives: Pippin Prep

KRAS-dependent sorting of miRNA to exosomes

Posted on September 27, 2015 by Alex

July 2015 Authors: Diana J Cha, Jeffrey L Franklin, Yongchao Dou, Qi Liu, James N Higginbotham, Michelle Demory Beckler, Alissa M Weaver, Kasey Vickers, Nirpesh Prasad, Shawn Levy, Bing Zhang, Robert J Coffey, and James G Patton Info: Scientists from … more »

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Biomarker discovery: quantification of microRNAs and other small non-coding RNAs using next generation sequencing

Posted on July 23, 2015 by Alex

July 2015 Authors: Juan Pablo Lopez, Alpha Diallo, Cristiana Cruceanu, Laura M. Fiori, Sylvie Laboissiere, Isabelle Guillet, Joelle Fontaine, Jiannis Ragoussis, Vladimir Benes, Gustavo Turecki, and Carl Ernst Info: Scientists from McGill University and the European Molecular Biology Laboratory evaluated … more »

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Arterial endothelial methylome: differential DNA methylation in athero-susceptible disturbed flow regions in vivo

Posted on July 23, 2015 by Alex

July 2015 Authors: Yi-Zhou Jiang, Elisabetta Manduchi, Christian J. Stoeckert, and Peter F. Davies Info: In this paper, scientists from the University of Pennsylvania used complete methylated DNA immunoprecipitation sequencing (MeDIP-seq) to study methylation differences in endothelial cells to find … more »

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Towards allele-level human leucocyte antigens genotyping – assessing two next-generation sequencing platforms: Ion Torrent Personal Genome Machine and Illumina MiSeq

Posted on July 2, 2015 by Alex

June 2015 Authors: J. L. Duke, C. Lind, K. Mackiewicz, D. Ferriola, A. Papazoglou, O. Derbeneva, D. Wallace, and D. S. Monos Info: This publication in the International Journal of Immunogenetics from scientists at the Children’s Hospital of Philadelphia presents … more »

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At Einstein Med School, ‘Extremely Reproducible’ Pippin Platforms Save Time and Money

Posted on May 19, 2015 by Alex

In the genetics department at Albert Einstein College of Medicine, Research Assistant Professor Alex Maslov is working to understand structural variants associated with aging and cancer. Using human and mouse cells, he deploys whole genome sequencing to make these links. … more »

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Double-digest RAD sequencing using Ion Proton semiconductor platform (ddRADseq-ion) with nonmodel organisms

Posted on May 13, 2015 by Alex

April 2015 Authors: Hans Recknagel, Arne Jacobs, Pawel Herzyk, Kathryn R Elmer Info: In this Molecular Ecology Resources paper, researchers from the University of Glasgow describe a protocol and adapters they developed for double-digest RAD sequencing on the Ion Torrent … more »

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Improved transposon-based library preparation for the Ion Torrent platform

Posted on April 21, 2015 by Alex

April 2015 Authors: Tatyana Gorbacheva, Wilber Quispe-Tintaya, Vasily N. Popov, Jan Vijg, and Alexander Y. Maslov Info: In this Biotechniques paper, scientists from the Albert Einstein College of Medicine in New York describe MuPlus, a new transposon-based method for barcoded … more »

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Video Protocol: NGS-based Methylation Mapping with Pippin Prep

Posted on April 21, 2015 by Alex

We always love a great protocol video, and this one from scientists at Weill Cornell Medical College, published through the Journal of Visualized Experiments, is a keeper. Check it out here: “Enhanced Reduced Representation Bisulfite Sequencing for Assessment of DNA … more »

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PippinHT in the Wild: High-Throughput ChIP-Seq at Whitehead Core Lab

Posted on April 20, 2015 by Alex

At the Whitehead Institute’s Genome Technology Core, scientists handle a lot of ChIP-seq and RNA-seq projects. To boost capacity in library prep, they recently upgraded from a small fleet of Pippin Prep instruments to the new PippinHT for high-throughput, automated … more »

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Discovery of Novel Rhabdoviruses in the Blood of Healthy Individuals from West Africa

Posted on April 13, 2015 by Alex

March 2015 Authors: Matthew H Stremlau, Kristian G Andersen, Onikepe A Folarin, Jessica N Grove, Ikponmwonsa Odia, Philomena E Ehiane, Omowunmi Omoniwa, Omigie Omoregie, Pan-Pan Jiang, Nathan L Yozwiak, Christian B Matranga, Xiao Yang, Stephen K Gire, Sarah Winnicki, Ridhi … more »

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