Improved transposon-based library preparation for the Ion Torrent platform

April 2015

Tatyana Gorbacheva, Wilber Quispe-Tintaya, Vasily N. Popov, Jan Vijg, and Alexander Y. Maslov

In this Biotechniques paper, scientists from the Albert Einstein College of Medicine in New York describe MuPlus, a new transposon-based method for barcoded library prep compatible with Ion Torrent sequencers. The protocol eliminates self-complementary regions, increasing sequencing efficiency. Pippin Prep was used for automated size selection of a mouse library.

BioTechniques, Vol. 58, No. 4, April 2015, pp. 200–202

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