Double-digest RAD sequencing using Ion Proton semiconductor platform (ddRADseq-ion) with nonmodel organisms

April 2015

Hans Recknagel, Arne Jacobs, Pawel Herzyk, Kathryn R Elmer

In this Molecular Ecology Resources paper, researchers from the University of Glasgow describe a protocol and adapters they developed for double-digest RAD sequencing on the Ion Torrent platform. They found that ddRADseq-ion’s strongest applications are pilot assessments and quick data return. They used Sage Pippin Prep to perform individual size selection from multiplexed libraries.

doi: 10.1111/1755-0998.12406

This entry was posted in Citation and tagged , , . Bookmark the permalink.

Comments are closed.