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Recent Posts
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- Identifying the best PCR enzyme for library amplification in NGS
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Tag Archives: Pippin Prep
Whole Genome Amplification and Reduced-Representation Genome Sequencing of Schistosoma japonicum Miracidia
January 2017 Authors: Jonathan A. Shortt, Daren C. Card, Drew R. Schield, Yang Liu, Bo Zhong, Todd A. Castoe, Elizabeth J. Carlton, and David D. Pollock Info: Researchers used ddRAD-seq to study the schistosome species S. japonicum miracidia, finding that … more »
Posted in Citation
Tagged ddRADseq, MiSeq, Pippin Prep
Comments Off on Whole Genome Amplification and Reduced-Representation Genome Sequencing of Schistosoma japonicum Miracidia
QseC inhibition as an antivirulence approach for colitis-associated bacteria
January 2017 Authors: Michelle G. Rooks, Patrick Veiga, Analise Z. Reeves, Sydney Lavoie, et al. Info: In this paper, scientists from Harvard and other institutions describe a study of quorum-sensing regulators in E. coli as a possible means of reducing … more »
Posted in Citation
Tagged MiSeq, Pippin Prep
Comments Off on QseC inhibition as an antivirulence approach for colitis-associated bacteria
Defining an olfactory receptor function in airway smooth muscle cells
December 2016 Authors: William H. Aisenberg, Jessie Huang, Wanqu Zhu, Premraj Rajkumar, et al. Info: Scientists at Johns Hopkins University, Rutgers, and other institutions teamed up to study receptors in airway smooth muscle cells that appear to play a role … more »
Posted in Citation
Tagged HiSeq, Pippin Prep, RNA-seq
Comments Off on Defining an olfactory receptor function in airway smooth muscle cells
The mammalian LINC complex regulates genome transcriptional responses to substrate rigidity
December 2016 Authors: Samer G. Alam, Qiao Zhang, Nripesh Prasad, Yuan Li, Srikar Chamala, et al. Info: Researchers from the HudsonAlpha Institute, the University of Florida, and other institutions studied the role of rigidity in the extracellular matrix, finding that … more »
Posted in Citation
Tagged microRNA, Pippin Prep
Comments Off on The mammalian LINC complex regulates genome transcriptional responses to substrate rigidity
HLA Typing Workflow Uses Pippin Prep for Size Selection
We’ve written before about the shift toward NGS-based technologies for the HLA typing market. HLA typing is used for everything from understanding autoimmune and infectious diseases to matching organ transplants to recipients. But the HLA locus really make scientists and … more »
Posted in Blog
Tagged HLA, Pippin Prep
Comments Off on HLA Typing Workflow Uses Pippin Prep for Size Selection
Generation of Full-Length Class I Human Leukocyte Antigen Gene Consensus Sequences for Novel Allele Characterization
December 2016 Authors: Peter M. Clark, Jamie L. Duke, Deborah Ferriola, Valia Bravo-Egana, Tunde Vago, Aniqa Hassan, Anna Papazoglou, and Dimitri Monos Info: Scientists from The Children’s Hospital of Philadelphia evaluated an NGS-based HLA typing kit from Omixon. They analyzed … more »
Posted in Citation
Tagged HLA, MiSeq, Pippin Prep
Comments Off on Generation of Full-Length Class I Human Leukocyte Antigen Gene Consensus Sequences for Novel Allele Characterization
Depletion of tRNA-halves enables effective small RNA sequencing of low-input murine serum samples
November 2016 Authors: Alan Van Goethem, Nurten Yigit, Celine Everaert, Myrthala Moreno-Smith, et al. Info: Scientists at Ghent University and Baylor College of Medicine evaluated protocols for depleting abundant RNAs from blood and serum samples to improve the ability to … more »
Posted in Citation
Tagged microRNA, Pippin Prep
Comments Off on Depletion of tRNA-halves enables effective small RNA sequencing of low-input murine serum samples
Structure of the germline genome of Tetrahymena thermophila and relationship to the massively rearranged somatic genome
November 2016 Authors: Eileen P Hamilton, Aurélie Kapusta, Piroska E Huvos, Shelby L Bidwell, et al. Info: A large team of scientists in Asia, Europe, and the U.S. collaborated for this study of Tetrahymena thermophile, for which they produced a complete sequence assembly. They … more »
Posted in Citation
Tagged HiSeq, Pippin Prep
Comments Off on Structure of the germline genome of Tetrahymena thermophila and relationship to the massively rearranged somatic genome
Association of MicroRNAs with Antibody Response to Mycoplasma bovis in Beef Cattle
August 2016 Authors: Eduardo Casas, Guohong Cai, Larry A. Kuehn, Karen B. Register, Tara G. McDaneld, John D. Neill Info: USDA scientists performed a longitudinal study of young cows to identify microRNAs linked to a serum antibody produced after exposure … more »
Posted in Citation
Tagged HiSeq, Illumina, microRNA, Pippin Prep, RNA sequencing
Comments Off on Association of MicroRNAs with Antibody Response to Mycoplasma bovis in Beef Cattle
Comparative genomics and transcriptomics of Pichia pastoris
August 2016 Authors: Kerry R. Love, Kartik A. Shah, Charles A. Whittaker, Jie Wu, M. Catherine Bartlett, Duanduan Ma, Rachel L. Leeson, Margaret Priest, Jonathan Borowsky, Sarah K. Young and J. Christopher Love Info: To provide a foundation for engineering … more »
Posted in Citation
Tagged DNA Sequencing, HiSeq, Illumina, Pippin Prep
Comments Off on Comparative genomics and transcriptomics of Pichia pastoris