Comprehensive evaluation of extracellular small RNA isolation methods from serum in high throughput sequencing

January 2017

Yan Guo, Kasey Vickers, Yanhua Xiong, Shilin Zhao, Quanhu Sheng, Pan Zhang, Wanding Zhou and Charles R. Flynn

Scientists from Vanderbilt University and Van Andel Research Institute compared methods for isolating circulating extracellular small non-coding RNAs. They used five kits on samples taken from one healthy individual. RNA-seq was performed on an Illumina NextSeq 500 following size selection with Pippin Prep.

BMC Genomics201718:50
DOI: 10.1186/s12864-016-3470-z

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