Whole Genome Amplification and Reduced-Representation Genome Sequencing of Schistosoma japonicum Miracidia

January 2017

Jonathan A. Shortt, Daren C. Card, Drew R. Schield, Yang Liu, Bo Zhong, Todd A. Castoe, Elizabeth J. Carlton, and David D. Pollock

Researchers used ddRAD-seq to study the schistosome species S. japonicum miracidia, finding that they could analyze up to 30,000 loci from archived samples. The approach offers significantly more information than microsatellite genotyping and is cost-effective. They used Pippin Prep to size-select libraries prior to sequencing on an Illumina MiSeq instrument.

PLoS Neglected Tropical Diseases

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