Tag Archives: PacBio

Capture of complete ciliate chromosomes in single sequencing reads reveals widespread chromosome isoforms

Posted on January 5, 2020 by Alex

December 2019 Authors: Kelsi A. Lindblad, Jananan S. Pathmanathan, Sandrine Moreira, John R. Bracht, Robert P. Sebra, Elizabeth R. Hutton & Laura F. Landweber Info: Researchers sequenced the whole genome of Oxytricha trifallax, a model protozoan ciliate, using long read … more »

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Comparison of mitochondrial DNA variants detection using short- and long-read sequencing

Posted on August 19, 2019 by Alex

August 2019 Authors: Ahmed N. Alkanaq, Kohei Hamanaka, Futoshi Sekiguchi, Masataka Taguri, Atsushi Takata, Noriko Miyake, Satoko Miyatake, Takeshi Mizuguchi, Naomichi Matsumot Info: The genotypes of mitochondrial DNA variants are compared using both short-read (Illumina Hi-SeqX10) and long-read (PacBio Sequel) … more »

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SageELF Size Selection for PacBio Circular Consensus Sequencing

Posted on January 17, 2019 by Alex

A new preprint has landed on BioRxiv that reports on high-accuracy circular consensus sequencing (CCS) on the PacBio Sequel. The study, “Highly-accurate long-read sequencing improves variant detection and assembly of a human genome”, is authored by PacBio and an impressive … more »

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Highly sensitive detection of mutations in CHO cell recombinant DNA using multi‐parallel single molecule real‐time DNA sequencing

Posted on May 29, 2018 by Alex

June 2018 Authors: Joseph Cartwright, Karin Anderson, Joseph Longworth, Philip Lobb, and David James Info: To improve the quantitative assessment of recombinant DNA in cell lines, University of Sheffield scientists and collaborators developed a PacBio-based method for high-throughput rDNA sequence … more »

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A Validation Approach of an End-to-End Whole Genome Sequencing Workflow for Source Tracking of Listeria monocytogenes and Salmonella enterica

Posted on April 15, 2018 by Alex

March 2018 Authors: Anne-Catherine Portmann, Coralie Fournier, Johan Gimonet, Catherine Ngom-Bru, et al. Info: Scientists from Nestle Research Center and Nestle Institute of Health Sciences aimed to establish guidelines for whole-genome sequencing used in contamination investigations in the food industry. … more »

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Full-length mRNA sequencing uncovers a widespread coupling between transcription initiation and mRNA processing

Posted on April 12, 2018 by Alex

March 2018 Authors: Seyed Yahya Anvar, Guy Allard, Elizabeth Tseng, Gloria Sheynkman, et al. Info: Researchers from Leiden University Medical Center and other institutions studied the relationships between transcription initiation and splicing and polyadenylation events in breast cancer cells and … more »

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The DNA Methylome of the Hyperthermoacidophilic Crenarchaeon Sulfolobus acidocaldarius

Posted on March 12, 2018 by Alex

February 2018 Authors: Mohea Couturier and Ann-Christin Lindås Info: Scientists from Stockholm University investigated the impact of DNA methylation in archaeal genomes by analyzing the complete DNA methylome of S. acidocaldarius using PacBio sequencing. The team used BluePippin to remove … more »

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Transcription-associated mutation promotes RNA complexity in highly expressed genes — a major new source of selectable variation

Posted on March 12, 2018 by Alex

February 2018 Authors: Shengkai Pan, Michael Bruford, Yusong Wang, Zhenzhen Lin, et al. Info: In this project, researchers studied transcriptomes in falcons, mice, and chickens and found that highly expressed genes are more likely to alternatively spliced. They also studied … more »

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Podcast: Blogger Keith Robison on the State of Sequencing

Posted on February 27, 2018 by Alex

A new podcast from Mendelspod takes a look at the state of sequencing in 2018. It’s a lively and interesting discussion about the current and future landscape of genomics between host Theral Timpson and Keith Robison, founder of the Omics! … more »

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SageELF Enables Targeted RenSeq Capture for PacBio Sequencing

Posted on February 13, 2018 by Alex

We’ve had targeted capture on the brain lately as we work with collaborators to put the finishing touches on our HLS-CATCH method for selecting large genomic elements using Cas9 guides and the SageHLS system. So today, we wanted to revisit … more »

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