Tag Archives: Illumina

Spotted in the Literature: The First PippinHT Publication!

Posted on May 9, 2016 by Alex

We began shipping the high-throughput version of our automated Pippin DNA size selection platform last year, and it’s a thrill to see what we believe is the first reference to it in a peer-reviewed publication. A team of scientists from … more »

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New hyRAD Method Expands Use of RAD-seq to Degraded Samples

Posted on March 31, 2016 by Alex

Since RAD-seq was first developed, we’ve seen a number of new versions and approaches from an enthusiastic scientific community. The latest was recently published in PLoS One and demonstrates a RAD-based method suitable for analyzing degraded DNA, an essential step … more »

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Identification of Human Islet Amyloid Polypeptide as a BACE2 Substrate

Posted on March 23, 2016 by Alex

February 2016 Authors: Ingrid C. Rulifson, Ping Cao, Li Miao, David Kopecky, Linda Huang, Ryan D. White, Kim Samayoa, Jonitha Gardner, Xiaosu Wu, Kui Chen, Trace Tsuruda, Oliver Homann, Helene Baribault, Harvey Yamane, Tim Carlson, Jed Wiltzius, Yang Li Info: … more »

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Comparison of Sample Preparation Methods Used for the Next-Generation Sequencing of Mycobacterium tuberculosis

Posted on March 22, 2016 by Alex

February 2016 Authors: Andrea D. Tyler, Sara Christianson, Natalie C. Knox, Philip Mabon, Joyce Wolfe, Gary Van Domselaar, Morag R. Graham, and Meenu K. Sharma Info: In this PLoS One publication, scientists in Manitoba compare the quality of libraries constructed … more »

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Biomarker discovery: quantification of microRNAs and other small non-coding RNAs using next generation sequencing

Posted on July 23, 2015 by Alex

July 2015 Authors: Juan Pablo Lopez, Alpha Diallo, Cristiana Cruceanu, Laura M. Fiori, Sylvie Laboissiere, Isabelle Guillet, Joelle Fontaine, Jiannis Ragoussis, Vladimir Benes, Gustavo Turecki, and Carl Ernst Info: Scientists from McGill University and the European Molecular Biology Laboratory evaluated … more »

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Arterial endothelial methylome: differential DNA methylation in athero-susceptible disturbed flow regions in vivo

Posted on July 23, 2015 by Alex

July 2015 Authors: Yi-Zhou Jiang, Elisabetta Manduchi, Christian J. Stoeckert, and Peter F. Davies Info: In this paper, scientists from the University of Pennsylvania used complete methylated DNA immunoprecipitation sequencing (MeDIP-seq) to study methylation differences in endothelial cells to find … more »

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Towards allele-level human leucocyte antigens genotyping – assessing two next-generation sequencing platforms: Ion Torrent Personal Genome Machine and Illumina MiSeq

Posted on July 2, 2015 by Alex

June 2015 Authors: J. L. Duke, C. Lind, K. Mackiewicz, D. Ferriola, A. Papazoglou, O. Derbeneva, D. Wallace, and D. S. Monos Info: This publication in the International Journal of Immunogenetics from scientists at the Children’s Hospital of Philadelphia presents … more »

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Modified Mate-Pair Protocol Lowers Cost, Boosts Library Yield

Posted on June 11, 2015 by Alex

Regular readers of the Sage Science blog know that we can never resist a good methods paper. We enjoyed this publication from the phyloinformatics group at RIKEN detailing a modified protocol for generating high-quality mate-pair libraries while significantly reducing costs. … more »

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Fecal virome analysis of three carnivores reveals a novel nodavirus and multiple gemycircularviruses

Posted on June 4, 2015 by Alex

May 2015 Authors: Nádia Conceição-Neto, Mark Zeller, Elisabeth Heylen, Hanne Lefrère, João Rodrigo Mesquita and Jelle Matthijnssens Info: This publication in Virology Journal describes metagenomics analyses of fecal samples from badger, mongoose, and otter to better understand viral populations in … more »

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Optimization and cost-saving in tagmentation-based mate-pair library preparation and sequencing

Posted on June 3, 2015 by Alex

May 2015 Authors: Kaori Tatsumi, Osamu Nishimura, Kazu Itomi, Chiharu Tanegashima, and Shigehiro Kuraku Info: Scientists from RIKEN modified the recommended protocol for the Nextera Mate Pair Sample Prep Kit from Illumina to improve library yield and quality while minimizing … more »

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