Optimization and cost-saving in tagmentation-based mate-pair library preparation and sequencing

May 2015

Kaori Tatsumi, Osamu Nishimura, Kazu Itomi, Chiharu Tanegashima, and Shigehiro Kuraku

Scientists from RIKEN modified the recommended protocol for the Nextera Mate Pair Sample Prep Kit from Illumina to improve library yield and quality while minimizing cost. Changes included optimizing tagmentation conditions, intensive shearing of DNA, and more. The team also switched the order of size selection, using BluePippin before the strand displacement reaction, which saves money by reducing reagent volumes.

BioTechniques 58:253-257 (May 2015)
doi 10.2144/000114288

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