Small-seq for single-cell small-RNA sequencing

September 2018

Michael Hagemann-Jensen, Ilgar Abdullayev, Rickard Sandberg and Omid R Faridani

A new protocol from researchers at the Karolinska Institutet and the Ludwig Institute for Cancer Research outline a method for making small RNA sequencing libraries from a single mammalian cell. The method increases sensitivity compared with other methods by using a oligonucleotides to mask ribosomal RNA rather than using a pulldown strategy. Pippin Prep is recommended to isolated miRNA away from other small RNAs.

Nature Protocols 13, pages2407–2424 (2018)

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